Loss of striatal somatostatin neurons following prenatal methylazoxymethanol

S. R. Vincent; K. Semba; J. M. Radke; A. Jakubovic; H. C. Fibiger

doi:10.1016/0014-4886(90)90030-V

Loss of striatal somatostatin neurons following prenatal methylazoxymethanol

S. R. Vincent, K. Semba, J. M. Radke, A. Jakubovic, H. C. Fibiger

Medicine

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Prenatal administration of methylazoxymethanol acetate (MAM), which kills neuroblasts undergoing mitosis, was used to lesion striatal somatostatin neurons. Previous [³H]thymidine autoradiographic studies had indicated that striatal somatostatin neurons undergo their final mitotic division at Gestational Days (G) 15 and 16. Therefore, pregnant Sprague-Dawley rats received an intraperitoneal injection of MAM (25 mg/kg) on G15. Neurochemical and histological examination of the mature offspring indicated the loss of half the striatal aspiny interneurons in which somatostatin, neuropeptide Y, and NADPH diaphorase coexist, with relative sparing of the cholinergic interneurons and medium spiny projection cells. This prenatal MAM treatment was without apparent effect on the patchmatrix organization of the striatum.

Original language	English
Pages (from-to)	194-200
Number of pages	7
Journal	Experimental Neurology
Volume	110
Issue number	2
DOIs	https://doi.org/10.1016/0014-4886(90)90030-V
Publication status	Published - Nov 1990

Bibliographical note

Funding Information:
This research was supported by grants from the Medical Research Council of Canada. S.R.V. is an MRC Scientist, and K.S. an MRC Scholar.

ASJC Scopus Subject Areas

Neurology
Developmental Neuroscience

Access to Document

10.1016/0014-4886(90)90030-V

Cite this

@article{a85559cebe2d4c6c999d53230d7d6189,

title = "Loss of striatal somatostatin neurons following prenatal methylazoxymethanol",

abstract = "Prenatal administration of methylazoxymethanol acetate (MAM), which kills neuroblasts undergoing mitosis, was used to lesion striatal somatostatin neurons. Previous [3H]thymidine autoradiographic studies had indicated that striatal somatostatin neurons undergo their final mitotic division at Gestational Days (G) 15 and 16. Therefore, pregnant Sprague-Dawley rats received an intraperitoneal injection of MAM (25 mg/kg) on G15. Neurochemical and histological examination of the mature offspring indicated the loss of half the striatal aspiny interneurons in which somatostatin, neuropeptide Y, and NADPH diaphorase coexist, with relative sparing of the cholinergic interneurons and medium spiny projection cells. This prenatal MAM treatment was without apparent effect on the patchmatrix organization of the striatum.",

author = "Vincent, {S. R.} and K. Semba and Radke, {J. M.} and A. Jakubovic and Fibiger, {H. C.}",

note = "Funding Information: This research was supported by grants from the Medical Research Council of Canada. S.R.V. is an MRC Scientist, and K.S. an MRC Scholar.",

year = "1990",

month = nov,

doi = "10.1016/0014-4886(90)90030-V",

language = "English",

volume = "110",

pages = "194--200",

journal = "Experimental Neurology",

issn = "0014-4886",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Loss of striatal somatostatin neurons following prenatal methylazoxymethanol

AU - Vincent, S. R.

AU - Semba, K.

AU - Radke, J. M.

AU - Jakubovic, A.

AU - Fibiger, H. C.

N1 - Funding Information: This research was supported by grants from the Medical Research Council of Canada. S.R.V. is an MRC Scientist, and K.S. an MRC Scholar.

PY - 1990/11

Y1 - 1990/11

N2 - Prenatal administration of methylazoxymethanol acetate (MAM), which kills neuroblasts undergoing mitosis, was used to lesion striatal somatostatin neurons. Previous [3H]thymidine autoradiographic studies had indicated that striatal somatostatin neurons undergo their final mitotic division at Gestational Days (G) 15 and 16. Therefore, pregnant Sprague-Dawley rats received an intraperitoneal injection of MAM (25 mg/kg) on G15. Neurochemical and histological examination of the mature offspring indicated the loss of half the striatal aspiny interneurons in which somatostatin, neuropeptide Y, and NADPH diaphorase coexist, with relative sparing of the cholinergic interneurons and medium spiny projection cells. This prenatal MAM treatment was without apparent effect on the patchmatrix organization of the striatum.

AB - Prenatal administration of methylazoxymethanol acetate (MAM), which kills neuroblasts undergoing mitosis, was used to lesion striatal somatostatin neurons. Previous [3H]thymidine autoradiographic studies had indicated that striatal somatostatin neurons undergo their final mitotic division at Gestational Days (G) 15 and 16. Therefore, pregnant Sprague-Dawley rats received an intraperitoneal injection of MAM (25 mg/kg) on G15. Neurochemical and histological examination of the mature offspring indicated the loss of half the striatal aspiny interneurons in which somatostatin, neuropeptide Y, and NADPH diaphorase coexist, with relative sparing of the cholinergic interneurons and medium spiny projection cells. This prenatal MAM treatment was without apparent effect on the patchmatrix organization of the striatum.

UR - http://www.scopus.com/inward/record.url?scp=0025203718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025203718&partnerID=8YFLogxK

U2 - 10.1016/0014-4886(90)90030-V

DO - 10.1016/0014-4886(90)90030-V

M3 - Article

C2 - 1977608

AN - SCOPUS:0025203718

SN - 0014-4886

VL - 110

SP - 194

EP - 200

JO - Experimental Neurology

JF - Experimental Neurology

IS - 2

ER -

Loss of striatal somatostatin neurons following prenatal methylazoxymethanol

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Access to Document

Other files and links

Fingerprint

Cite this