Measurement of 32P following colorimetric phosphorus analysis

F. B.St C. Palmer

doi:10.1016/0003-2697(69)90292-9

Measurement of ³²P following colorimetric phosphorus analysis

F. B.St C. Palmer

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A procedure for the determination of both ³²P and phosphate contents on the same sample has been described. Following phosphate estimation by the method of Bartlett (5), the solution containing the blue reduced phosphomolybdate complex is decolorized at high pH. ³²P is measured in this transparent aqueous solution with a liquid scintillation spectrometer, without the addition of scintillators, by utilizing the Čerenkov radiation.

Original language	English
Pages (from-to)	493-501
Number of pages	9
Journal	Analytical Biochemistry
Volume	31
Issue number	C
DOIs	https://doi.org/10.1016/0003-2697(69)90292-9
Publication status	Published - 1969

Bibliographical note

Funding Information:
F. Cameron is gratefully acknowledged. from the Medical Research Council of

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

PubMed: MeSH publication types

Journal Article

Access to Document

10.1016/0003-2697(69)90292-9

Cite this

@article{626d82b14d944b92adb6037781c66502,

title = "Measurement of 32P following colorimetric phosphorus analysis",

abstract = "A procedure for the determination of both 32P and phosphate contents on the same sample has been described. Following phosphate estimation by the method of Bartlett (5), the solution containing the blue reduced phosphomolybdate complex is decolorized at high pH. 32P is measured in this transparent aqueous solution with a liquid scintillation spectrometer, without the addition of scintillators, by utilizing the {\v C}erenkov radiation.",

author = "Palmer, {F. B.St C.}",

note = "Funding Information: F. Cameron is gratefully acknowledged. from the Medical Research Council of",

year = "1969",

doi = "10.1016/0003-2697(69)90292-9",

language = "English",

volume = "31",

pages = "493--501",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "C",

}

TY - JOUR

T1 - Measurement of 32P following colorimetric phosphorus analysis

AU - Palmer, F. B.St C.

N1 - Funding Information: F. Cameron is gratefully acknowledged. from the Medical Research Council of

PY - 1969

Y1 - 1969

N2 - A procedure for the determination of both 32P and phosphate contents on the same sample has been described. Following phosphate estimation by the method of Bartlett (5), the solution containing the blue reduced phosphomolybdate complex is decolorized at high pH. 32P is measured in this transparent aqueous solution with a liquid scintillation spectrometer, without the addition of scintillators, by utilizing the Čerenkov radiation.

AB - A procedure for the determination of both 32P and phosphate contents on the same sample has been described. Following phosphate estimation by the method of Bartlett (5), the solution containing the blue reduced phosphomolybdate complex is decolorized at high pH. 32P is measured in this transparent aqueous solution with a liquid scintillation spectrometer, without the addition of scintillators, by utilizing the Čerenkov radiation.

UR - http://www.scopus.com/inward/record.url?scp=0014593314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014593314&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(69)90292-9

DO - 10.1016/0003-2697(69)90292-9

M3 - Article

C2 - 5382629

AN - SCOPUS:0014593314

SN - 0003-2697

VL - 31

SP - 493

EP - 501

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - C

ER -