Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace

Daniela Chessa; Manuela Murgia; Emanuela Sias; Massimo Deligios; Vittorio Mazzarello; Maura Fiamma; Daniela Rovina; Gabriele Carenti; Giulia Ganau; Elisabetta Pintore; Mauro Fiori; Gemma L. Kay; Alessandro Ponzeletti; Piero Cappuccinelli; David J. Kelvin; John Wain; Salvatore Rubino

doi:10.1038/s41598-020-69497-8

Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace

Daniela Chessa, Manuela Murgia, Emanuela Sias, Massimo Deligios, Vittorio Mazzarello, Maura Fiamma, Daniela Rovina, Gabriele Carenti, Giulia Ganau, Elisabetta Pintore, Mauro Fiori, Gemma L. Kay, Alessandro Ponzeletti, Piero Cappuccinelli, David J. Kelvin, John Wain, Salvatore Rubino

Medicine

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

This study evidenced the presence of parasites in a cesspit of an aristocratic palace of nineteenth century in Sardinia (Italy) by the use of classical paleoparasitological techniques coupled with next-generation sequencing. Parasite eggs identified by microscopy included helminth genera pathogenic for humans and animals: the whipworm Trichuris sp., the roundworm Ascaris sp., the flatworm Dicrocoelium sp. and the fish tapeworm Diphyllobothrium sp. In addition, 18S rRNA metabarcoding and metagenomic sequencing analysis allowed the first description in Sardinia of aDNA of the human specific T. trichiura species and Ascaris genus. Their presence is important for understanding the health conditions, hygiene habits, agricultural practices and the diet of the local inhabitants in the period under study.

Original language	English
Article number	12656
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-69497-8
Publication status	Published - Dec 1 2020

Bibliographical note

Funding Information:
This work was supported by the Li-Ka Shing Foundation, Shantou University Medical College; Dalhousie Medical Research Foundation; and a Rapid Response Award from Research Nova Scotia. D.J.K. holds the Canada Research Chair in Translational Vaccinology and Inflammation. We would like to thank the consultation of Prof. Giovanni Garippa from the Department of Veterinary Medicine of the University of Sassari and Prof. Maria Teresa Manfredi from the Department of Veterinary Medicine of the University of Milan.

Publisher Copyright:
© 2020, The Author(s).

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Chessa, D., Murgia, M., Sias, E., Deligios, M., Mazzarello, V., Fiamma, M., Rovina, D., Carenti, G., Ganau, G., Pintore, E., Fiori, M., Kay, G. L., Ponzeletti, A., Cappuccinelli, P., Kelvin, D. J., Wain, J., & Rubino, S. (2020). Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace. Scientific Reports, 10(1), Article 12656. https://doi.org/10.1038/s41598-020-69497-8

Chessa, D, Murgia, M, Sias, E, Deligios, M, Mazzarello, V, Fiamma, M, Rovina, D, Carenti, G, Ganau, G, Pintore, E, Fiori, M, Kay, GL, Ponzeletti, A, Cappuccinelli, P, Kelvin, DJ, Wain, J & Rubino, S 2020, 'Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace', Scientific Reports, vol. 10, no. 1, 12656. https://doi.org/10.1038/s41598-020-69497-8

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Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

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