MIP-1a, rantes, and IP-10 chemokine expression is associated with T-Cell infiltration during cardiac allograft rejection

L. Ran, R. Rahimpour, J. Jiang, J. Wang, B. Garcia, R. Zhong, D. J. Kelvin

Research output: Contribution to journalArticlepeer-review

Abstract

We hypothesized that chemokines are pivital molecules for the recruitment of leukocytes during graft rejection. To explore this idea we examined the expression of chemokines in the C57B1/6 to Balb/c heterotopic heart allograft transplant model. Histologkal scores indicated that mild rejection was observed as early as day 3, moderate on day 5 and severe on day 7 in allografted hearts. Immunohistochemical staining (ISH) indicated that CD4 and CDS positive T cells were present as early as day 3 and steadily increased in numbers on days 5 and 7 in the allografted hearts but were rarely observed in isografts. ISH staining for chemokines paralleled the observations of mononuclear infiltration. In allograft tissue, MIP-la, MIP-lb, and RANTES, all potent chemoattractants for monocytes and T cells, were detected as early as day 3 but the number of positive cells was greatly increased on days 5 and 7. RT-PCR on isolated RNA showed that MIP-lb, RANTES and IP-10 were expressed in allografted hearts on days 3, 5 and 7 but were not expressed in native hearts and isografts on any days; MCP-1 was detected in some but not all isografts on days 1, 3 and 5. Our data indicates MIP-la, MIP-lb, and RANTES chemokine expression is correlated with, and perhaps responsible for, T cell and mononuclear infiltration during the early stages of allograft rejection.

Original languageEnglish
Pages (from-to)A1024
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996

ASJC Scopus Subject Areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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