TY - JOUR
T1 - MIP-1a, rantes, and IP-10 chemokine expression is associated with T-Cell infiltration during cardiac allograft rejection
AU - Ran, L.
AU - Rahimpour, R.
AU - Jiang, J.
AU - Wang, J.
AU - Garcia, B.
AU - Zhong, R.
AU - Kelvin, D. J.
PY - 1996
Y1 - 1996
N2 - We hypothesized that chemokines are pivital molecules for the recruitment of leukocytes during graft rejection. To explore this idea we examined the expression of chemokines in the C57B1/6 to Balb/c heterotopic heart allograft transplant model. Histologkal scores indicated that mild rejection was observed as early as day 3, moderate on day 5 and severe on day 7 in allografted hearts. Immunohistochemical staining (ISH) indicated that CD4 and CDS positive T cells were present as early as day 3 and steadily increased in numbers on days 5 and 7 in the allografted hearts but were rarely observed in isografts. ISH staining for chemokines paralleled the observations of mononuclear infiltration. In allograft tissue, MIP-la, MIP-lb, and RANTES, all potent chemoattractants for monocytes and T cells, were detected as early as day 3 but the number of positive cells was greatly increased on days 5 and 7. RT-PCR on isolated RNA showed that MIP-lb, RANTES and IP-10 were expressed in allografted hearts on days 3, 5 and 7 but were not expressed in native hearts and isografts on any days; MCP-1 was detected in some but not all isografts on days 1, 3 and 5. Our data indicates MIP-la, MIP-lb, and RANTES chemokine expression is correlated with, and perhaps responsible for, T cell and mononuclear infiltration during the early stages of allograft rejection.
AB - We hypothesized that chemokines are pivital molecules for the recruitment of leukocytes during graft rejection. To explore this idea we examined the expression of chemokines in the C57B1/6 to Balb/c heterotopic heart allograft transplant model. Histologkal scores indicated that mild rejection was observed as early as day 3, moderate on day 5 and severe on day 7 in allografted hearts. Immunohistochemical staining (ISH) indicated that CD4 and CDS positive T cells were present as early as day 3 and steadily increased in numbers on days 5 and 7 in the allografted hearts but were rarely observed in isografts. ISH staining for chemokines paralleled the observations of mononuclear infiltration. In allograft tissue, MIP-la, MIP-lb, and RANTES, all potent chemoattractants for monocytes and T cells, were detected as early as day 3 but the number of positive cells was greatly increased on days 5 and 7. RT-PCR on isolated RNA showed that MIP-lb, RANTES and IP-10 were expressed in allografted hearts on days 3, 5 and 7 but were not expressed in native hearts and isografts on any days; MCP-1 was detected in some but not all isografts on days 1, 3 and 5. Our data indicates MIP-la, MIP-lb, and RANTES chemokine expression is correlated with, and perhaps responsible for, T cell and mononuclear infiltration during the early stages of allograft rejection.
UR - http://www.scopus.com/inward/record.url?scp=33748899293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748899293&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33748899293
SN - 0892-6638
VL - 10
SP - A1024
JO - FASEB Journal
JF - FASEB Journal
IS - 6
ER -