Modulation of protective immunity, eosinophilia, and cytokine responses by selective mutagenesis of a recombinant G protein vaccine against respiratory syncytial virus

Yan Huang, Robert Anderson

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Using an Escherichia coli-grown plasmid vector encoding a fragment of thioredoxin (Trx) fused to a central region (amino acids 128 to 229) of the respiratory syncytial virus (RSV) (Long strain) G protein, we employed site-directed mutagenesis to investigate the importance of selected amino acids to vaccine efficacy. Mice were immunized with a total of 10 wild-type or mutant Trx-G proteins and challenged intranasally with RSV. Striking differences in the induction of RSV G-protein-specific antibodies, protection against RSV challenge, cytokine RNA responses, and induction of RSV-associated eosinophilic inflammation were observed among the mutant proteins examined. Taken together, the results identify a critical role for specific amino acids in the induction of protective immunity and priming for eosinophilia against RSV.

Original languageEnglish
Pages (from-to)4527-4532
Number of pages6
JournalJournal of Virology
Volume79
Issue number7
DOIs
Publication statusPublished - Apr 2005

ASJC Scopus Subject Areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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