Abstract
Textiles containing interposed layers of polyethylene oxide (PEO)-fibrinogen and PEO-thrombin fibers are explored as biomaterials for hemostasis. The PEO-fibrinogen and PEO-thrombin fibers are formed by contact drawing, an approach that uses an entangled polymer solution and a pin array to form fibers by extension of liquid bridges. The interposition of the PEO-fibrinogen and PEO-thrombin fibers results in polymerization of a fibrin hydrogel mesh once the textile is hydrated. This fibrin hydrogel mesh displays the expected bands and diffraction peaks by Fourier-transform infrared spectromicroscopy and X-ray diffraction, respectively. The functionality of the hemostatic textiles formed from the interposed PEO-fibrinogen and PEO-thrombin fibers is demonstrated by analyzing human blood hemolysis, complement activation, protein adsorption, and platelet and leukocyte adhesion, indicating compatibility with human blood (hemolysis ratio <5%), with minimal inflammatory response (levels of terminal complement complex equivalent to plasma). The cytocompatibility and potential for cell remodeling of the fibrin hydrogel mesh formed by this process is evaluated with human dermal fibroblasts and human keratinocytes and it is found that both cell types attach and grow on the fibrin mesh. Finally, a whole blood clotting time of less than 30 s suggests a potential use of this material in hemorrhage control.
Original language | English |
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Journal | Advanced Materials Interfaces |
DOIs | |
Publication status | Accepted/In press - 2022 |
Bibliographical note
Funding Information:The authors acknowledge use of the XRD system in the laboratory of Dr. David N. Langelaan at Dalhousie University (Canada Foundation for Innovation and Research Nova Scotia Project #36853). The authors also acknowledge use of the ATR‐FTIR system in the laboratory of Dr. Jan Rainey at Dalhousie University (Natural Sciences and Engineering Research Council of Canada RTI/000030‐2020). This work was supported by funds from the Canada Research Chairs Program (J.P.F.), the Canada Foundation for Innovation (J.P.F., Project #33533), the Natural Sciences and Engineering Research Council of Canada (J.P.F., RGPIN/04298‐2016; L.K., RGPIN/03781‐2018), and the New Frontiers in Research Fund (J.P.F. and L.K., NFRFE/2018‐00356). S.K.V. acknowledges salary support from a Killam Postdoctoral Fellowship. The authors acknowledge that Dalhousie University is located in Mi'kma'ki, the ancestral and unceded territory of the Mi'kmaq.
Publisher Copyright:
© 2022 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.
ASJC Scopus Subject Areas
- Mechanics of Materials
- Mechanical Engineering