Nucleoplasmin-Mediated Decondensation of Mytilus Sperm Chromatin. Identification and Partial Characterization of a Nucleoplasmin-like Protein with Sperm-Nuclei Decondensing Activity in Mytilus califomianus

Philip Rice; Rafael Garduno; Tom Itoh; Chiaki Katagiri; Juan Ausio

doi:10.1021/bi00023a001

Nucleoplasmin-Mediated Decondensation of Mytilus Sperm Chromatin. Identification and Partial Characterization of a Nucleoplasmin-like Protein with Sperm-Nuclei Decondensing Activity in Mytilus califomianus

Philip Rice, Rafael Garduno, Tom Itoh, Chiaki Katagiri, Juan Ausio

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We have been able to induce sperm nuclear decondensation in the mussel Mytilus califomianus (mollusc) using either egg extracts or pure nucleoplasmin from Xenopus (amphibian). The nuclear decondensation involves removal of the sperm nuclear basic proteins (SNBPs) which bind to nucleoplasmin. An attempt has been made to isolate an ooplasmic factor from Mytilus with a similar sperm-chromatin decondensing activity. An acidic, thermostable protein with a molecular mass of 58 000 has been purified and partially characterized.

Original language	English
Pages (from-to)	7563-7568
Number of pages	6
Journal	Biochemistry
Volume	34
Issue number	23
DOIs	https://doi.org/10.1021/bi00023a001
Publication status	Published - Jun 1995
Externally published	Yes

ASJC Scopus Subject Areas

Biochemistry

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Nucleoplasmin-Mediated Decondensation of Mytilus Sperm Chromatin. Identification and Partial Characterization of a Nucleoplasmin-like Protein with Sperm-Nuclei Decondensing Activity in Mytilus califomianus. / Rice, Philip; Garduno, Rafael; Itoh, Tom et al.
In: Biochemistry, Vol. 34, No. 23, 06.1995, p. 7563-7568.

Research output: Contribution to journal › Article › peer-review

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abstract = "We have been able to induce sperm nuclear decondensation in the mussel Mytilus califomianus (mollusc) using either egg extracts or pure nucleoplasmin from Xenopus (amphibian). The nuclear decondensation involves removal of the sperm nuclear basic proteins (SNBPs) which bind to nucleoplasmin. An attempt has been made to isolate an ooplasmic factor from Mytilus with a similar sperm-chromatin decondensing activity. An acidic, thermostable protein with a molecular mass of 58 000 has been purified and partially characterized.",

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year = "1995",

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AU - Itoh, Tom

AU - Katagiri, Chiaki

AU - Ausio, Juan

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Nucleoplasmin-Mediated Decondensation of Mytilus Sperm Chromatin. Identification and Partial Characterization of a Nucleoplasmin-like Protein with Sperm-Nuclei Decondensing Activity in Mytilus califomianus

Abstract

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