Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio)

Marianne Pierce, Yimin Wang, Eileen M. Denovan-Wright, Jonathan M. Wright

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

We have cloned a cDNA from zebrafish (Danio rerio) that contains an open-reading frame of 132 amino acids coding for a fatty acid binding protein (FABP) of approximately 15 kDa. Multiple sequence alignment revealed extensive amino acid identity between this zebrafish FABP and intestinal-like FABPs (I-FABP) from other species. The zebrafish I-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases PstI, BglII or EcoRI suggesting that a single copy of the I-FABP gene is present in the zebrafish genome. An oligonucleotide probe complementary to the zebrafish I-FABP mRNA hybridized to an mRNA of approximately 800 bases in Northern blot analysis. In situ hybridization revealed that the I-FABP mRNA was expressed exclusively in the intestine of the adult zebrafish. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1490
Issue number1-2
DOIs
Publication statusPublished - Jan 31 2000

Bibliographical note

Funding Information:
We wish to thank Dr. D. Grunwald (University of Utah, USA) for kindly providing the zebrafish λZAP II cDNA libraries and Lorraine Hamilton for the zebrafish RNA. This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to J.M.W. Y.W. was a recipient of a Killam postdoctoral fellowship from Dalhousie University.

ASJC Scopus Subject Areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Fingerprint

Dive into the research topics of 'Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio)'. Together they form a unique fingerprint.

Cite this

Pierce, M., Wang, Y., Denovan-Wright, E. M., & Wright, J. M. (2000). Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio). Biochimica et Biophysica Acta - Gene Structure and Expression, 1490(1-2), 175-183. https://doi.org/10.1016/S0167-4781(99)00229-8