Paratrypanosoma is a novel early-branching trypanosomatid

Pavel Flegontov; Jan Votýpka; Tomáš Skalický; Maria D. Logacheva; Aleksey A. Penin; Goro Tanifuji; Naoko T. Onodera; Alexey S. Kondrashov; Petr Volf; John M. Archibald; Julius Lukeš

doi:10.1016/j.cub.2013.07.045

Paratrypanosoma is a novel early-branching trypanosomatid

Pavel Flegontov, Jan Votýpka, Tomáš Skalický, Maria D. Logacheva, Aleksey A. Penin, Goro Tanifuji, Naoko T. Onodera, Alexey S. Kondrashov, Petr Volf, John M. Archibald, Julius Lukeš

Medicine

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

The kinetoplastids are a widespread and important group of single-celled eukaryotes, many of which are devastating parasites of animals, including humans [1-3]. We have discovered a new insect trypanosomatid in the gut of Culex pipiens mosquitoes. Glyceraldehyde-3-phosphate dehydrogenase- and SSU rRNA-based phylogenetic analyses show this parasite to constitute a distinct branch between the free-living Bodo saltans and the obligatory parasitic clades represented by the genus Trypanosoma and other trypanosomatids. From draft genome sequence data, we identified 114 protein genes shared among the new flagellate, 15 trypanosomatid species, B. saltans, and the heterolobosean Naegleria gruberi, as well as 129 protein genes shared with the basal kinetoplastid Perkinsela sp. Individual protein phylogenies together with analyses of concatenated alignments show that the new species, here named Paratrypanosoma confusum n. gen., n. sp., branches with very high support at the base of the family Trypanosomatidae. P. confusum thus represents a long-sought-after missing link between the ancestral free-living bodonids and the derived parasitic trypanosomatids. Further analysis of the P. confusum genome should provide insight into the emergence of parasitism in the medically important trypanosomatids.

Original language	English
Pages (from-to)	1787-1793
Number of pages	7
Journal	Current Biology
Volume	23
Issue number	18
DOIs	https://doi.org/10.1016/j.cub.2013.07.045
Publication status	Published - Sept 23 2013

Bibliographical note

Funding Information:
The help of Eva Suková with photodocumentation, Aleš Horák with phylogenetic analyses, and Milena Svobodová and Jana Rádrová with isolation and PCR detection of flagellates in mosquito hosts is appreciated. This work was supported by the Czech Grant Agency (P305/11/2179, 206/09/H026) and a Praemium Academiae award to J.L., who is also a Fellow of the Canadian Institute for Advanced Research (CIFAR). Research in the Archibald Lab was supported by an operating grant from the Canadian Institutes of Health Research (CIHR). J.M.A. holds a CIHR New Investigator Award and is a CIFAR Fellow. M.D.L., A.A.P., A.S.K. were supported by Russian Ministry of Education and Science (G34.31.0008). The access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum, provided under the program LM2010005, is appreciated. The access to the CERIT-SC computing and storage facilities provided under the program Center CERIT Scientific Cloud, part of the Operational Program Research and Development for Innovations, regulation number CZ. 1.05/3.2.00/08.0144, is acknowledged.

ASJC Scopus Subject Areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences

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title = "Paratrypanosoma is a novel early-branching trypanosomatid",

abstract = "The kinetoplastids are a widespread and important group of single-celled eukaryotes, many of which are devastating parasites of animals, including humans [1-3]. We have discovered a new insect trypanosomatid in the gut of Culex pipiens mosquitoes. Glyceraldehyde-3-phosphate dehydrogenase- and SSU rRNA-based phylogenetic analyses show this parasite to constitute a distinct branch between the free-living Bodo saltans and the obligatory parasitic clades represented by the genus Trypanosoma and other trypanosomatids. From draft genome sequence data, we identified 114 protein genes shared among the new flagellate, 15 trypanosomatid species, B. saltans, and the heterolobosean Naegleria gruberi, as well as 129 protein genes shared with the basal kinetoplastid Perkinsela sp. Individual protein phylogenies together with analyses of concatenated alignments show that the new species, here named Paratrypanosoma confusum n. gen., n. sp., branches with very high support at the base of the family Trypanosomatidae. P. confusum thus represents a long-sought-after missing link between the ancestral free-living bodonids and the derived parasitic trypanosomatids. Further analysis of the P. confusum genome should provide insight into the emergence of parasitism in the medically important trypanosomatids.",

author = "Pavel Flegontov and Jan Vot{\'y}pka and Tom{\'a}{\v s} Skalick{\'y} and Logacheva, {Maria D.} and Penin, {Aleksey A.} and Goro Tanifuji and Onodera, {Naoko T.} and Kondrashov, {Alexey S.} and Petr Volf and Archibald, {John M.} and Julius Luke{\v s}",

note = "Funding Information: The help of Eva Sukov{\'a} with photodocumentation, Ale{\v s} Hor{\'a}k with phylogenetic analyses, and Milena Svobodov{\'a} and Jana R{\'a}drov{\'a} with isolation and PCR detection of flagellates in mosquito hosts is appreciated. This work was supported by the Czech Grant Agency (P305/11/2179, 206/09/H026) and a Praemium Academiae award to J.L., who is also a Fellow of the Canadian Institute for Advanced Research (CIFAR). Research in the Archibald Lab was supported by an operating grant from the Canadian Institutes of Health Research (CIHR). J.M.A. holds a CIHR New Investigator Award and is a CIFAR Fellow. M.D.L., A.A.P., A.S.K. were supported by Russian Ministry of Education and Science (G34.31.0008). The access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum, provided under the program LM2010005, is appreciated. The access to the CERIT-SC computing and storage facilities provided under the program Center CERIT Scientific Cloud, part of the Operational Program Research and Development for Innovations, regulation number CZ. 1.05/3.2.00/08.0144, is acknowledged. ",

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AU - Flegontov, Pavel

AU - Votýpka, Jan

AU - Skalický, Tomáš

AU - Logacheva, Maria D.

AU - Penin, Aleksey A.

AU - Tanifuji, Goro

AU - Onodera, Naoko T.

AU - Kondrashov, Alexey S.

AU - Volf, Petr

AU - Archibald, John M.

AU - Lukeš, Julius

N1 - Funding Information: The help of Eva Suková with photodocumentation, Aleš Horák with phylogenetic analyses, and Milena Svobodová and Jana Rádrová with isolation and PCR detection of flagellates in mosquito hosts is appreciated. This work was supported by the Czech Grant Agency (P305/11/2179, 206/09/H026) and a Praemium Academiae award to J.L., who is also a Fellow of the Canadian Institute for Advanced Research (CIFAR). Research in the Archibald Lab was supported by an operating grant from the Canadian Institutes of Health Research (CIHR). J.M.A. holds a CIHR New Investigator Award and is a CIFAR Fellow. M.D.L., A.A.P., A.S.K. were supported by Russian Ministry of Education and Science (G34.31.0008). The access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum, provided under the program LM2010005, is appreciated. The access to the CERIT-SC computing and storage facilities provided under the program Center CERIT Scientific Cloud, part of the Operational Program Research and Development for Innovations, regulation number CZ. 1.05/3.2.00/08.0144, is acknowledged.

PY - 2013/9/23

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N2 - The kinetoplastids are a widespread and important group of single-celled eukaryotes, many of which are devastating parasites of animals, including humans [1-3]. We have discovered a new insect trypanosomatid in the gut of Culex pipiens mosquitoes. Glyceraldehyde-3-phosphate dehydrogenase- and SSU rRNA-based phylogenetic analyses show this parasite to constitute a distinct branch between the free-living Bodo saltans and the obligatory parasitic clades represented by the genus Trypanosoma and other trypanosomatids. From draft genome sequence data, we identified 114 protein genes shared among the new flagellate, 15 trypanosomatid species, B. saltans, and the heterolobosean Naegleria gruberi, as well as 129 protein genes shared with the basal kinetoplastid Perkinsela sp. Individual protein phylogenies together with analyses of concatenated alignments show that the new species, here named Paratrypanosoma confusum n. gen., n. sp., branches with very high support at the base of the family Trypanosomatidae. P. confusum thus represents a long-sought-after missing link between the ancestral free-living bodonids and the derived parasitic trypanosomatids. Further analysis of the P. confusum genome should provide insight into the emergence of parasitism in the medically important trypanosomatids.

AB - The kinetoplastids are a widespread and important group of single-celled eukaryotes, many of which are devastating parasites of animals, including humans [1-3]. We have discovered a new insect trypanosomatid in the gut of Culex pipiens mosquitoes. Glyceraldehyde-3-phosphate dehydrogenase- and SSU rRNA-based phylogenetic analyses show this parasite to constitute a distinct branch between the free-living Bodo saltans and the obligatory parasitic clades represented by the genus Trypanosoma and other trypanosomatids. From draft genome sequence data, we identified 114 protein genes shared among the new flagellate, 15 trypanosomatid species, B. saltans, and the heterolobosean Naegleria gruberi, as well as 129 protein genes shared with the basal kinetoplastid Perkinsela sp. Individual protein phylogenies together with analyses of concatenated alignments show that the new species, here named Paratrypanosoma confusum n. gen., n. sp., branches with very high support at the base of the family Trypanosomatidae. P. confusum thus represents a long-sought-after missing link between the ancestral free-living bodonids and the derived parasitic trypanosomatids. Further analysis of the P. confusum genome should provide insight into the emergence of parasitism in the medically important trypanosomatids.

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Paratrypanosoma is a novel early-branching trypanosomatid

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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