PEG and mPEG-anthracene induce DNA condensation and particle formation

E. Froehlich; J. S. Mandeville; D. Arnold; L. Kreplak; H. A. Tajmir-Riahi

doi:10.1021/jp205079u

PEG and mPEG-anthracene induce DNA condensation and particle formation

E. Froehlich, J. S. Mandeville, D. Arnold, L. Kreplak, H. A. Tajmir-Riahi

Medicine

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

In this study, we investigated the binding of DNA with poly(ethylene glycol) (PEG) of different sizes and compositions such as PEG 3350, PEG 6000, and mPEG-anthracene in aqueous solution at physiological conditions. The effects of size and composition on DNA aggregation and condensation as well as conformation were determined using Fourier transform infrared (FTIR), UV-visible, CD, fluorescence spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed moderate complex formation for PEG 3350 and PEG 6000 and weaker interaction for mPE-anthracene-DNA adducts with both hydrophilic and hydrophobic contacts. The order of ± stability of the complexes formed is K_{PEG 6000} = 1.5 (±0.4) × 10 ⁴ M^-1 > K_{PEG 3350} = 7.9 (±1) × 10³ M^-1 > K_{mPEG-anthracene}= 3.6 (±0.8) × 10³ M^-1 with nearly 1 bound PEG molecule per DNA. No B-DNA conformational changes were observed, while DNA condensation and particle formation occurred at high PEG concentration.

Original language	English
Pages (from-to)	9873-9879
Number of pages	7
Journal	Journal of Physical Chemistry B
Volume	115
Issue number	32
DOIs	https://doi.org/10.1021/jp205079u
Publication status	Published - Aug 18 2011

ASJC Scopus Subject Areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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@article{645ce516b8724afeb98845d8d671b776,

title = "PEG and mPEG-anthracene induce DNA condensation and particle formation",

abstract = "In this study, we investigated the binding of DNA with poly(ethylene glycol) (PEG) of different sizes and compositions such as PEG 3350, PEG 6000, and mPEG-anthracene in aqueous solution at physiological conditions. The effects of size and composition on DNA aggregation and condensation as well as conformation were determined using Fourier transform infrared (FTIR), UV-visible, CD, fluorescence spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed moderate complex formation for PEG 3350 and PEG 6000 and weaker interaction for mPE-anthracene-DNA adducts with both hydrophilic and hydrophobic contacts. The order of ± stability of the complexes formed is KPEG 6000 = 1.5 (±0.4) × 10 4 M-1 > KPEG 3350 = 7.9 (±1) × 103 M-1 > KmPEG-anthracene= 3.6 (±0.8) × 103 M-1 with nearly 1 bound PEG molecule per DNA. No B-DNA conformational changes were observed, while DNA condensation and particle formation occurred at high PEG concentration.",

author = "E. Froehlich and Mandeville, {J. S.} and D. Arnold and L. Kreplak and Tajmir-Riahi, {H. A.}",

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doi = "10.1021/jp205079u",

language = "English",

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journal = "Journal of Physical Chemistry B",

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TY - JOUR

T1 - PEG and mPEG-anthracene induce DNA condensation and particle formation

AU - Froehlich, E.

AU - Mandeville, J. S.

AU - Arnold, D.

AU - Kreplak, L.

AU - Tajmir-Riahi, H. A.

PY - 2011/8/18

Y1 - 2011/8/18

N2 - In this study, we investigated the binding of DNA with poly(ethylene glycol) (PEG) of different sizes and compositions such as PEG 3350, PEG 6000, and mPEG-anthracene in aqueous solution at physiological conditions. The effects of size and composition on DNA aggregation and condensation as well as conformation were determined using Fourier transform infrared (FTIR), UV-visible, CD, fluorescence spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed moderate complex formation for PEG 3350 and PEG 6000 and weaker interaction for mPE-anthracene-DNA adducts with both hydrophilic and hydrophobic contacts. The order of ± stability of the complexes formed is KPEG 6000 = 1.5 (±0.4) × 10 4 M-1 > KPEG 3350 = 7.9 (±1) × 103 M-1 > KmPEG-anthracene= 3.6 (±0.8) × 103 M-1 with nearly 1 bound PEG molecule per DNA. No B-DNA conformational changes were observed, while DNA condensation and particle formation occurred at high PEG concentration.

AB - In this study, we investigated the binding of DNA with poly(ethylene glycol) (PEG) of different sizes and compositions such as PEG 3350, PEG 6000, and mPEG-anthracene in aqueous solution at physiological conditions. The effects of size and composition on DNA aggregation and condensation as well as conformation were determined using Fourier transform infrared (FTIR), UV-visible, CD, fluorescence spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed moderate complex formation for PEG 3350 and PEG 6000 and weaker interaction for mPE-anthracene-DNA adducts with both hydrophilic and hydrophobic contacts. The order of ± stability of the complexes formed is KPEG 6000 = 1.5 (±0.4) × 10 4 M-1 > KPEG 3350 = 7.9 (±1) × 103 M-1 > KmPEG-anthracene= 3.6 (±0.8) × 103 M-1 with nearly 1 bound PEG molecule per DNA. No B-DNA conformational changes were observed, while DNA condensation and particle formation occurred at high PEG concentration.

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DO - 10.1021/jp205079u

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SN - 1520-6106

VL - 115

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EP - 9879

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

IS - 32

ER -

PEG and mPEG-anthracene induce DNA condensation and particle formation

Abstract

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