Percoll purification of chromaffin granules inhibits their ability to take up and maintain calcium

Peter G. Jones, Joel Meneses, David M. Waisman

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Secretory granules of the adrenal medulla have recently been shown to be able to sequester and release Ca2+, in addition to their previously established role as carriers of secretory products. In order to study the ability of these or any other secretory granules to participate in intracellular calcium homeostasis, it is imperative that they should be free of other contaminating Ca2+ sequestering organelles, and that the Ca2+ uptake and release mechanisms of those granules should remain intact throughout any chosen purification procedure. We report here that chromaffin granules which were purified by the isopycnic gradient medium Percoll, or even incubated with it, showed an attenuated ability to sequester Ca2+.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalFEBS Letters
Volume336
Issue number2
DOIs
Publication statusPublished - Dec 27 1993
Externally publishedYes

ASJC Scopus Subject Areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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