Protein σ1 is the reovirus cell attachment protein

Patrick W.K. Lee; Edward C. Hayes; Wolfgang K. Joklik

doi:10.1016/0042-6822(81)90535-3

Protein σ1 is the reovirus cell attachment protein

Patrick W.K. Lee, Edward C. Hayes, Wolfgang K. Joklik

Research output: Contribution to journal › Article › peer-review

245 Citations (Scopus)

Abstract

The minor outer capsid shell component σ1 is the reovirus cell attachment protein because (a) IgGs directed against σ1 prevent reovirus particle adsorption, (b) free protein σ1 present in lysates of infected cells is capable of adsorbing to cells, and (c) σ1 competes with reovirus particles for cell surface receptors. Competition experiments indicate that reovirus particles of all three serotypes attach to the same cell surface receptor. It is also shown that σ1 is located on the surface of reovirus particles in close juxtaposition to λ2, the major, if not the only, component of the 12 icosahedrally distributed reovirus core projections or spikes, which penetrate through the outer capsid shell to the reovirus particle surface.

Original language	English
Pages (from-to)	156-163
Number of pages	8
Journal	Virology
Volume	108
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(81)90535-3
Publication status	Published - Jan 15 1981
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Research Grants ROI AI 08909 and R01 AI 10132 and by Research Training Grant T32 CA 09111 from the NIH. P. W. K. Lee was the recipient of a Medical Research Council of Canada Fellowship. We would like to acknowledge with gratitude the expert technical assistance of Sherry Montgomery.

ASJC Scopus Subject Areas

Virology

PubMed: MeSH publication types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

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10.1016/0042-6822(81)90535-3

Cite this

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title = "Protein σ1 is the reovirus cell attachment protein",

abstract = "The minor outer capsid shell component σ1 is the reovirus cell attachment protein because (a) IgGs directed against σ1 prevent reovirus particle adsorption, (b) free protein σ1 present in lysates of infected cells is capable of adsorbing to cells, and (c) σ1 competes with reovirus particles for cell surface receptors. Competition experiments indicate that reovirus particles of all three serotypes attach to the same cell surface receptor. It is also shown that σ1 is located on the surface of reovirus particles in close juxtaposition to λ2, the major, if not the only, component of the 12 icosahedrally distributed reovirus core projections or spikes, which penetrate through the outer capsid shell to the reovirus particle surface.",

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note = "Funding Information: This work was supported by Research Grants ROI AI 08909 and R01 AI 10132 and by Research Training Grant T32 CA 09111 from the NIH. P. W. K. Lee was the recipient of a Medical Research Council of Canada Fellowship. We would like to acknowledge with gratitude the expert technical assistance of Sherry Montgomery.",

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AU - Hayes, Edward C.

AU - Joklik, Wolfgang K.

N1 - Funding Information: This work was supported by Research Grants ROI AI 08909 and R01 AI 10132 and by Research Training Grant T32 CA 09111 from the NIH. P. W. K. Lee was the recipient of a Medical Research Council of Canada Fellowship. We would like to acknowledge with gratitude the expert technical assistance of Sherry Montgomery.

PY - 1981/1/15

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N2 - The minor outer capsid shell component σ1 is the reovirus cell attachment protein because (a) IgGs directed against σ1 prevent reovirus particle adsorption, (b) free protein σ1 present in lysates of infected cells is capable of adsorbing to cells, and (c) σ1 competes with reovirus particles for cell surface receptors. Competition experiments indicate that reovirus particles of all three serotypes attach to the same cell surface receptor. It is also shown that σ1 is located on the surface of reovirus particles in close juxtaposition to λ2, the major, if not the only, component of the 12 icosahedrally distributed reovirus core projections or spikes, which penetrate through the outer capsid shell to the reovirus particle surface.

AB - The minor outer capsid shell component σ1 is the reovirus cell attachment protein because (a) IgGs directed against σ1 prevent reovirus particle adsorption, (b) free protein σ1 present in lysates of infected cells is capable of adsorbing to cells, and (c) σ1 competes with reovirus particles for cell surface receptors. Competition experiments indicate that reovirus particles of all three serotypes attach to the same cell surface receptor. It is also shown that σ1 is located on the surface of reovirus particles in close juxtaposition to λ2, the major, if not the only, component of the 12 icosahedrally distributed reovirus core projections or spikes, which penetrate through the outer capsid shell to the reovirus particle surface.

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Protein σ1 is the reovirus cell attachment protein

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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