Removal of gram-negative endotoxin from solutions by affinity chromatography

Andrew C. Issekutz

doi:10.1016/0022-1759(83)90221-1

Removal of gram-negative endotoxin from solutions by affinity chromatography

Andrew C. Issekutz

Medicine

Research output: Contribution to journal › Article › peer-review

158 Citations (Scopus)

Abstract

Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process is described which exploits the high binding affinity of polymyxin B for the lipid A moiety of most endotoxins in order to remove endotoxins from solutions by chromatography on polymyxin B Sepharose 4B. This method was simple, very effective, resulting in essentially complete removal of several endotoxins from heavily contaminated solutions (1-10 μg/ml by Limulus amoebocyte lysate assay) and employed mild physiological conditions.

Original language	English
Pages (from-to)	275-281
Number of pages	7
Journal	Journal of Immunological Methods
Volume	61
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(83)90221-1
Publication status	Published - Jul 29 1983

Bibliographical note

Funding Information:
The author wishes to acknowledge the technical help of M/'. M. Ripley and the excellent secretarial assistance of Ms. R. Sampson. A. Issekutz is supported in part by Medical Research Council of Canada Grant DG-209. The work was supported by M.R.C. Grants DG-211 and MA-7684

ASJC Scopus Subject Areas

Immunology and Allergy
Immunology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0022-1759(83)90221-1

Cite this

Issekutz, A. C. (1983). Removal of gram-negative endotoxin from solutions by affinity chromatography. Journal of Immunological Methods, 61(3), 275-281. https://doi.org/10.1016/0022-1759(83)90221-1

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AB - Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process is described which exploits the high binding affinity of polymyxin B for the lipid A moiety of most endotoxins in order to remove endotoxins from solutions by chromatography on polymyxin B Sepharose 4B. This method was simple, very effective, resulting in essentially complete removal of several endotoxins from heavily contaminated solutions (1-10 μg/ml by Limulus amoebocyte lysate assay) and employed mild physiological conditions.

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Removal of gram-negative endotoxin from solutions by affinity chromatography

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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