Restraint stress affects hippocampal cell proliferation differently in rats and mice

Megan J. Bain; Suzanne M. Dwyer; Benjamin Rusak

doi:10.1016/j.neulet.2004.04.096

Restraint stress affects hippocampal cell proliferation differently in rats and mice

Megan J. Bain, Suzanne M. Dwyer, Benjamin Rusak

Medicine

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

Granule cell neurogenesis occurs in the dentate gyrus of the mammalian hippocampus throughout adult life, and incorporation of bromodeoxyuridine (BrdU) into DNA can serve as a marker of cell division associated with such neurogenesis. We examined the effects of a stressor (3 h of restraint) on hippocampal cell proliferation in Sprague-Dawley rats and C57BL/6J mice. Animals were killed immediately following restraint stress and their brains were prepared for immunohistochemical studies. Restraint stress caused similar significant increases in c-Fos immunoreactivity among cells in the hypothalamic paraventricular nucleus of both species, indicating that the stress experienced was similar. The restraint procedure also caused a significant decrease in BrdU labeling in the dentate gyrus of rats, as previously reported, but a significant increase in the same region in mice. Hippocampal cell proliferation appears to respond differently to restraint stress in these species.

Original language	English
Pages (from-to)	7-10
Number of pages	4
Journal	Neuroscience Letters
Volume	368
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2004.04.096
Publication status	Published - Sept 16 2004

Bibliographical note

Funding Information:
We are grateful to Donna Goguen, Debbie Fice, Karthika Devarajan and Marc Goguen for their excellent technical assistance. Supported by a grant from NSERC of Canada (A0305) and fellowships from the Nova Scotia Health Research Foundation and the Canadian Institutes of Health Research/Wyeth.

ASJC Scopus Subject Areas

General Neuroscience

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10.1016/j.neulet.2004.04.096

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title = "Restraint stress affects hippocampal cell proliferation differently in rats and mice",

abstract = "Granule cell neurogenesis occurs in the dentate gyrus of the mammalian hippocampus throughout adult life, and incorporation of bromodeoxyuridine (BrdU) into DNA can serve as a marker of cell division associated with such neurogenesis. We examined the effects of a stressor (3 h of restraint) on hippocampal cell proliferation in Sprague-Dawley rats and C57BL/6J mice. Animals were killed immediately following restraint stress and their brains were prepared for immunohistochemical studies. Restraint stress caused similar significant increases in c-Fos immunoreactivity among cells in the hypothalamic paraventricular nucleus of both species, indicating that the stress experienced was similar. The restraint procedure also caused a significant decrease in BrdU labeling in the dentate gyrus of rats, as previously reported, but a significant increase in the same region in mice. Hippocampal cell proliferation appears to respond differently to restraint stress in these species.",

author = "Bain, {Megan J.} and Dwyer, {Suzanne M.} and Benjamin Rusak",

note = "Funding Information: We are grateful to Donna Goguen, Debbie Fice, Karthika Devarajan and Marc Goguen for their excellent technical assistance. Supported by a grant from NSERC of Canada (A0305) and fellowships from the Nova Scotia Health Research Foundation and the Canadian Institutes of Health Research/Wyeth.",

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AU - Rusak, Benjamin

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N2 - Granule cell neurogenesis occurs in the dentate gyrus of the mammalian hippocampus throughout adult life, and incorporation of bromodeoxyuridine (BrdU) into DNA can serve as a marker of cell division associated with such neurogenesis. We examined the effects of a stressor (3 h of restraint) on hippocampal cell proliferation in Sprague-Dawley rats and C57BL/6J mice. Animals were killed immediately following restraint stress and their brains were prepared for immunohistochemical studies. Restraint stress caused similar significant increases in c-Fos immunoreactivity among cells in the hypothalamic paraventricular nucleus of both species, indicating that the stress experienced was similar. The restraint procedure also caused a significant decrease in BrdU labeling in the dentate gyrus of rats, as previously reported, but a significant increase in the same region in mice. Hippocampal cell proliferation appears to respond differently to restraint stress in these species.

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Restraint stress affects hippocampal cell proliferation differently in rats and mice

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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