Abstract
A restriction fragment length polymorphism study was undertaken to determine the extent and location of heterogeneity within spaP encoding the M(r) 185,000 cell surface protein P1 (antigen I/II) of Streptococcus mutans serotype c isolates. The gene was found to be highly conserved except for a central variable (V) region predicted to encode less than 150 amino acids. Sequence analysis identified two V-region variants. These differences were independent of the geographic source of the isolates. Southern analysis using synthetic oligonucleotide probes indicated that nonretention of P1 (I/II) by some isolates is not due to a deletion of the 3'-terminal DNA necessary to encode an intact carboxy terminus.
| Original language | English |
|---|---|
| Pages (from-to) | 1803-1810 |
| Number of pages | 8 |
| Journal | Infection and Immunity |
| Volume | 59 |
| Issue number | 5 |
| Publication status | Published - 1991 |
| Externally published | Yes |
ASJC Scopus Subject Areas
- Parasitology
- Microbiology
- Immunology
- Infectious Diseases
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Brady, L. J., Crowley, P. J., Ma, J. K. C., Kelly, C., Lee, S. F., Lehner, T., & Bleiweis, A. S. (1991). Restriction fragment length polymorphisms and sequence variation within the spaP gene of Streptococcus mutans serotype c isolates. Infection and Immunity, 59(5), 1803-1810.