Resveratrol, piperine and apigenin differ in their NADPH-oxidase inhibitory and reactive oxygen species-scavenging properties

Scott Whitehouse, Pei Lin Chen, Anna L. Greenshields, Mat Nightingale, David W. Hoskin, Karen Bedard

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Background Many plant-derived chemicals have been studied for their potential benefits in ailments including inflammation, cancer, neurodegeneration, and cardiovascular disease. The health benefits of phytochemicals are often attributed to the targeting of reactive oxygen species (ROS). However, it is not always clear whether these agents act directly as antioxidants to remove ROS, or whether they act indirectly by blocking ROS production by enzymes such as NADPH oxidase (NOX) enzymes, or by influencing the expression of cellular pro- and anti- oxidants. Hypothesis/Purpose Here we evaluate the pro- and anti-oxidant and NOX-inhibiting qualities of four phytochemicals: celastrol, resveratrol, apigenin, and piperine. Study Design This work was done using the H661 cell line expressing little or no NOX, modified H661 cells expressing NOX1 and its subunits, and an EBV-transformed B-lymphoblastoid cell line expressing endogenous NOX2. ROS were measured using Amplex Red and nitroblue tetrazolium assays. In addition, direct ROS scavenging of hydrogen peroxide or superoxide generated were measured using Amplex Red and methyl cypridina luciferin analog (MCLA). Results Of the four plant-derived compounds evaluated, only celastrol displayed NOX inhibitory activities, while celastrol and resveratrol both displayed ROS scavenging activity. Very little impact on ROS was observed with apigenin, or piperine. Conclusion The results of this study reveal the differences that exist between cell-free and intracellular pro-oxidant and antioxidant activities of several plant-derived compounds.

Original languageEnglish
Pages (from-to)1494-1503
Number of pages10
JournalPhytomedicine
Volume23
Issue number12
DOIs
Publication statusPublished - Nov 15 2016

Bibliographical note

Funding Information:
Funding for this study was provided by the Nova Scotia Health Research Foundation. The authors would like to thank Dr. Marie-Jose Stasia for kindly providing the control B-lymphocyte cell line used in this study, and Dr. Émilie Lefort and Dr. Jonathan Blay for helpful discussions and providing the apigenin.

Publisher Copyright:
© 2016 Elsevier GmbH

ASJC Scopus Subject Areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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