Secretoneurin and chemoattractant receptor interactions

C. Kong; B. M. Gill; R. Rahimpour; L. Xu; R. D. Feldman; Q. Xiao; T. J. McDonald; L. Taupenot; S. K. Mahata; B. Singh; D. T. O'Connor; D. J. Kelvin

doi:10.1016/S0165-5728(98)00094-0

Secretoneurin and chemoattractant receptor interactions

C. Kong, B. M. Gill, R. Rahimpour, L. Xu, R. D. Feldman, Q. Xiao, T. J. McDonald, L. Taupenot, S. K. Mahata, B. Singh, D. T. O'Connor, D. J. Kelvin

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Secretoneurin (SN) is a 33-amino acid peptide derived from secretogranin II (chromogranin C) which induces chemotaxis of monocytes but not neutrophils. In this study, we found that SN interacted with specific cell surface binding sites on human monocytes. The chemoattractants MCP-1, MCP-2 or fMLP could not compete for SN binding sites suggesting SN may bind to a novel chemotactic receptor. Additional studies showed that neither SN nor MCP-2 induced a rise in cytosolic Ca²⁺, and chemotaxis to SN was inhibited by cholera toxin (CT) and pertussis toxin (PT). Chemotactic desensitization studies demonstrated that fMLP, MCP-1, SN, and MCP-2 could all desensitize monocytes to subsequent SN stimulation. Our results indicate that SN binds to a cell surface receptor expressed on monocytes and activates signaling pathways which are sensitive to CT and PT.

Original language	English
Pages (from-to)	91-98
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	88
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(98)00094-0
Publication status	Published - Aug 1 1998
Externally published	Yes

Bibliographical note

Funding Information:
We thank Christine Moogk and J. Andrews for excellent technical assistance and Dr. S.J. Dixon (Dept. of Physiology, Univ. of Western Ontario) for assistance with fluorescence measurement of [Ca 2+ ] i . The authors also thank Mark DeVries for computer assistance. This work was supported by grants from the MRC, JDF-MRC, and Heart and Stroke of Canada.

ASJC Scopus Subject Areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0165-5728(98)00094-0

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title = "Secretoneurin and chemoattractant receptor interactions",

abstract = "Secretoneurin (SN) is a 33-amino acid peptide derived from secretogranin II (chromogranin C) which induces chemotaxis of monocytes but not neutrophils. In this study, we found that SN interacted with specific cell surface binding sites on human monocytes. The chemoattractants MCP-1, MCP-2 or fMLP could not compete for SN binding sites suggesting SN may bind to a novel chemotactic receptor. Additional studies showed that neither SN nor MCP-2 induced a rise in cytosolic Ca2+, and chemotaxis to SN was inhibited by cholera toxin (CT) and pertussis toxin (PT). Chemotactic desensitization studies demonstrated that fMLP, MCP-1, SN, and MCP-2 could all desensitize monocytes to subsequent SN stimulation. Our results indicate that SN binds to a cell surface receptor expressed on monocytes and activates signaling pathways which are sensitive to CT and PT.",

author = "C. Kong and Gill, {B. M.} and R. Rahimpour and L. Xu and Feldman, {R. D.} and Q. Xiao and McDonald, {T. J.} and L. Taupenot and Mahata, {S. K.} and B. Singh and O'Connor, {D. T.} and Kelvin, {D. J.}",

note = "Funding Information: We thank Christine Moogk and J. Andrews for excellent technical assistance and Dr. S.J. Dixon (Dept. of Physiology, Univ. of Western Ontario) for assistance with fluorescence measurement of [Ca 2+ ] i . The authors also thank Mark DeVries for computer assistance. This work was supported by grants from the MRC, JDF-MRC, and Heart and Stroke of Canada.",

year = "1998",

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doi = "10.1016/S0165-5728(98)00094-0",

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AU - Kong, C.

AU - Gill, B. M.

AU - Rahimpour, R.

AU - Xu, L.

AU - Feldman, R. D.

AU - Xiao, Q.

AU - McDonald, T. J.

AU - Taupenot, L.

AU - Mahata, S. K.

AU - Singh, B.

AU - O'Connor, D. T.

AU - Kelvin, D. J.

N1 - Funding Information: We thank Christine Moogk and J. Andrews for excellent technical assistance and Dr. S.J. Dixon (Dept. of Physiology, Univ. of Western Ontario) for assistance with fluorescence measurement of [Ca 2+ ] i . The authors also thank Mark DeVries for computer assistance. This work was supported by grants from the MRC, JDF-MRC, and Heart and Stroke of Canada.

PY - 1998/8/1

Y1 - 1998/8/1

N2 - Secretoneurin (SN) is a 33-amino acid peptide derived from secretogranin II (chromogranin C) which induces chemotaxis of monocytes but not neutrophils. In this study, we found that SN interacted with specific cell surface binding sites on human monocytes. The chemoattractants MCP-1, MCP-2 or fMLP could not compete for SN binding sites suggesting SN may bind to a novel chemotactic receptor. Additional studies showed that neither SN nor MCP-2 induced a rise in cytosolic Ca2+, and chemotaxis to SN was inhibited by cholera toxin (CT) and pertussis toxin (PT). Chemotactic desensitization studies demonstrated that fMLP, MCP-1, SN, and MCP-2 could all desensitize monocytes to subsequent SN stimulation. Our results indicate that SN binds to a cell surface receptor expressed on monocytes and activates signaling pathways which are sensitive to CT and PT.

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Secretoneurin and chemoattractant receptor interactions

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

UN SDGs

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