Super-Resolution Radial Fluctuations (SRRF) Microscopy

Jayme Salsman; Graham Dellaire

doi:10.1007/978-1-0716-2051-9_14

Super-Resolution Radial Fluctuations (SRRF) Microscopy

Jayme Salsman, Graham Dellaire

Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Citations (Scopus)

Abstract

Super-resolution Radial Fluctuations (SRRF) imaging is a computational approach to fixed and live-cell super-resolution microscopy that is highly accessible to life science researchers since it uses common microscopes and open-source software plugins for ImageJ. This allows users to generate super-resolution images using the same equipment, fluorophores, fluorescent proteins and methods they routinely employ for their studies without specialized sample preparations or reagents. Here, we discuss a step-by-step workflow for acquiring and analyzing images using the NanoJ-SRRF software developed by the Ricardo Henriques group, with a focus on imaging chromatin. Increased accessibility of affordable super-resolution imaging techniques is an important step in extending the reach of this revolution in cellular imaging to a greater number of laboratories.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	225-251
Number of pages	27
DOIs	https://doi.org/10.1007/978-1-0716-2051-9_14
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2440
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
for microscope upgrades for SRRF imaging were funded by an Equipment Grant from the Dalhousie Medical Research Foundation (DMRF), and a Research, Tools, & Instruments (RTI) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC). We would also like to thank Nvidia Corporation for the gift of the Titan V GPU used in this study obtained through their Higher Education and Research grants program. We would also like to thank Dr. Ricardo Henriques (Instituto Gulbenkian de Ciência, Portugal and University College London, UK) and his research group for their advice and access to the development-stage NanoJ-LiveSRRF software. Finally, we thank Dr. Gražvydas Lukinavičius and Dr. Jonas Bucevičius (Research group for chromatin labeling and imaging, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany) for the generous gift of the 5-TMR-Hoechst DNA dye used in this chapter.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

Access to Document

10.1007/978-1-0716-2051-9_14

Cite this

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abstract = "Super-resolution Radial Fluctuations (SRRF) imaging is a computational approach to fixed and live-cell super-resolution microscopy that is highly accessible to life science researchers since it uses common microscopes and open-source software plugins for ImageJ. This allows users to generate super-resolution images using the same equipment, fluorophores, fluorescent proteins and methods they routinely employ for their studies without specialized sample preparations or reagents. Here, we discuss a step-by-step workflow for acquiring and analyzing images using the NanoJ-SRRF software developed by the Ricardo Henriques group, with a focus on imaging chromatin. Increased accessibility of affordable super-resolution imaging techniques is an important step in extending the reach of this revolution in cellular imaging to a greater number of laboratories.",

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Super-Resolution Radial Fluctuations (SRRF) Microscopy

Abstract

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Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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