Surface-disorganized, attenuated mutants of Aeromonas salmonicida as furunculosis live vaccines

A slow-growing, aminoglycoside-resistant mutant and a rapidly-growing psuedorevertant were isolated from Aeromonas salmonicida, the causative agent of salmonid furunculosis. These mutants continued to elicit a variety of classical virulence factors associated with A. salmonicida pathogenesis. They differed morphologically from the wild-type and from one another with respect to A-layer organization, membrane antagonist sensitivity and particularly to aerobic metabolism. Both mutants were drastically altered in the architecture of the 2D crystalline surface array (A-layer), although both were similar to wild-type with respect to cell surface composition. The slow-growing, antibiotic-resistant mutant differed significantly from the wildtype by the apparent loss of virtually all aerobic metabolism; the pseudorevertant had partially recovered the ability to aerobically metabolize certain carbon sources. Both mutants were avirulent and incapable of tissue persistence. The rapidly-growing, antibiotic-sensitive pseudorevertant, when administered either intraperitoneally or by immersion, effectively protected salmonid fish from challenge by a heterologous virulent strain suggesting its candidature as a live, attenuated furunculosis vaccine.