Susceptibility of human embryonic kidneys in organ culture to herpesvirus hominis

J. F.S. Crocker; J. A. Embil; K. B. Easterbrook; D. B. Stoltz; J. M. Sparling

doi:10.1159/000181371

Susceptibility of human embryonic kidneys in organ culture to herpesvirus hominis

J. F.S. Crocker, J. A. Embil, K. B. Easterbrook, D. B. Stoltz, J. M. Sparling

Medicine

Medicine

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Abstract

101 pairs of human embryonic kidneys of 5-12 weeks' gestation were maintained in whole organ culture by our previously described technique, with herpesvirus hominis types 1 and 2 added to our regular media. One kidney of each pair served as a control and was exposed to identical culture medium without virus. Cultures were maintained for 24-120 h to study the time sequence of viral infectivity. Organs were then examined for the presence of virus by electron microscopy and histochemical staining. Histological studies of virus-infected kidneys showed either (1) complete organ death or (2) virus localization in undifferentiated cells, plus disorganization of architecture in differentiated areas. Control organs showed normal organization.

Original language	English
Pages (from-to)	48-54
Number of pages	7
Journal	Unknown Journal
Volume	21
Issue number	1
DOIs	https://doi.org/10.1159/000181371
Publication status	Published - 1978

ASJC Scopus Subject Areas

Physiology
Nephrology
Physiology (medical)
Urology

PubMed: MeSH publication types

Journal Article

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10.1159/000181371

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abstract = "101 pairs of human embryonic kidneys of 5-12 weeks' gestation were maintained in whole organ culture by our previously described technique, with herpesvirus hominis types 1 and 2 added to our regular media. One kidney of each pair served as a control and was exposed to identical culture medium without virus. Cultures were maintained for 24-120 h to study the time sequence of viral infectivity. Organs were then examined for the presence of virus by electron microscopy and histochemical staining. Histological studies of virus-infected kidneys showed either (1) complete organ death or (2) virus localization in undifferentiated cells, plus disorganization of architecture in differentiated areas. Control organs showed normal organization.",

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AU - Crocker, J. F.S.

AU - Embil, J. A.

AU - Easterbrook, K. B.

AU - Stoltz, D. B.

AU - Sparling, J. M.

PY - 1978

Y1 - 1978

N2 - 101 pairs of human embryonic kidneys of 5-12 weeks' gestation were maintained in whole organ culture by our previously described technique, with herpesvirus hominis types 1 and 2 added to our regular media. One kidney of each pair served as a control and was exposed to identical culture medium without virus. Cultures were maintained for 24-120 h to study the time sequence of viral infectivity. Organs were then examined for the presence of virus by electron microscopy and histochemical staining. Histological studies of virus-infected kidneys showed either (1) complete organ death or (2) virus localization in undifferentiated cells, plus disorganization of architecture in differentiated areas. Control organs showed normal organization.

AB - 101 pairs of human embryonic kidneys of 5-12 weeks' gestation were maintained in whole organ culture by our previously described technique, with herpesvirus hominis types 1 and 2 added to our regular media. One kidney of each pair served as a control and was exposed to identical culture medium without virus. Cultures were maintained for 24-120 h to study the time sequence of viral infectivity. Organs were then examined for the presence of virus by electron microscopy and histochemical staining. Histological studies of virus-infected kidneys showed either (1) complete organ death or (2) virus localization in undifferentiated cells, plus disorganization of architecture in differentiated areas. Control organs showed normal organization.

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Susceptibility of human embryonic kidneys in organ culture to herpesvirus hominis

Abstract

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