Abstract
A series of synthetic sialosides were evaluated for their ability to interact with reovirus serotype 3. It was found that siolosides with terminal N-acetylneuraminic acid (NeuNAc) linked in either an α2,3 or α2,6 configuration effectively blocked the binding of reovirus to mouse L fibroblasts, in contrast to a monosaccharide mixture containing the oligosaccharide constituents. Direct binding of reovirus to the sialosides was also demonstrable using sialosides conjugated to bovine serum albumin as ligands in a solid phase binding system. Of particular significance was the finding that the conjugate containing α-sialic acid alone (linked to bovine serum albumin) was capable of being recognized by reovirus at a level comparable to that of the other sialoside conjugates. Virus binding was abrogated by pretreating such conjugates with neuraminidase. These results suggest that the α-anomeric form of sialic acid is the minimal receptor determinant for reovirus recognition.
| Original language | English |
|---|---|
| Pages (from-to) | 382-385 |
| Number of pages | 4 |
| Journal | Virology |
| Volume | 172 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sept 1989 |
| Externally published | Yes |
Bibliographical note
Funding Information:We thank Dr. R. M. Ratcliffe (Chembiomed, Edmonton, Alberta) for supplying the sialosides and sialoslde-BSA conjugates and for helpful discusslons. This work was supported by the Medical Research Council of Canada. R.W.P. was a recipient of an Alberta Heritage Foundation for Medical Research (AHFMR) Studentship. A.H.C.C. is an AHFMR Fellow and P.W.K.L. is an AHFMR Scholar.
ASJC Scopus Subject Areas
- Virology