Abstract
Incubation of canine erythrocytes in 0.5% CO2, in air or 5% CO2 has no significant effect on glucose utilization if pH is constant. Decreasing pH from 7.7 to 7.2 decreased glucose utilization at a given concentration of CO2. At a given pH decreasing CO2 from 5% to 0.5% increased lactate production. Decreasing pH from 7.7 to 7.3 reduced potassium uptake by erythrocytes and decreasing CO2 from 5% to 0.5% decreased uptake further, so that net output was observed at pH 7.2, CO2 0.5%. These findings are consistent with a pH effect upon glucose utilization probably at the phosphofructokinase step. These data are suggestive of a second control step responsive to CO2 which lies distal to the pH effect. The effect of CO2 on potassium flux probably represents an alteration in erythrocyte energy metabolism with decrease energy availability. Hydrogen ion and CO2 control of erythrocyte metabolism could act to alter haemoglobin affinity for oxygen and carbon dioxide and hence act as a physiologic signal system.
Original language | English |
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Pages (from-to) | 96-103 |
Number of pages | 8 |
Journal | Respiration Physiology |
Volume | 15 |
Issue number | 1 |
DOIs | |
Publication status | Published - May 1972 |
Externally published | Yes |
Bibliographical note
Funding Information:Dr. D. T. Zborowska-Sluisw as supportedb y a fellowship from the Muscular Dystrophy Association of Canada and Dr. G. A. Klassen is a John and Mary R. Markle Scholar.
ASJC Scopus Subject Areas
- Physiology
- Pulmonary and Respiratory Medicine
PubMed: MeSH publication types
- Journal Article