Abstract
The observation that phosphatidyl inositol is incompletely extracted from liver tissue by chloroform-methanol mixtures containing a large proportion of water was confirmed. However, different metabolic turnover rates were not observed for the extractable and unextractable phosphatidyl inositol. It was then shown that acidic phospholipids are completely solubilized when the tissue is homogenized in chloroform-methanol but are readsorbed onto the insoluble tissue proteins if sufficient water is added to result in a two-phase system. This effect was observed in a synthetic system with both acidic and basic proteins and was found to be suppressed by low concentrations of divalent cations. Higher cation concentrations were required to suppress the readsorption of phosphatidyl inositol than the other acidic phospholipids. When tissue was extracted in this way the concentration of divalent cations originating from the tissue was adequate to suppress the readsorption of all acidic phospholipids except phosphatidyl inositol.
| Original language | English |
|---|---|
| Pages (from-to) | 134-144 |
| Number of pages | 11 |
| Journal | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids |
| Volume | 231 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 2 1971 |
Bibliographical note
Funding Information:This interpretation was supported by studies employing purified phospholipids and proteins. The behaviour of the major liver phospholipids in the presence of purified proteins was studied in a system consisting of 4 ml of chlorofo~-methanol (I : I, by vol.) to which a varying quantity of 0.05 M NaCl (0.0-5.0 ml) was added (Fig. I).
ASJC Scopus Subject Areas
- Biophysics
- Biochemistry
- Endocrinology