TY - JOUR
T1 - The human hematopoietic stem cell compartment is heterogeneous for CXCR4 expression
AU - Rosu-Myles, Michael
AU - Gallacher, Lisa
AU - Murdoch, Barbara
AU - Hess, David A.
AU - Keeney, Mike
AU - Kelvin, David
AU - Dale, Leanne
AU - Ferguson, Stephen S.G.
AU - Wu, Dongmei
AU - Fellows, Fraser
AU - Bhatia, Mickie
PY - 2000/12/19
Y1 - 2000/12/19
N2 - The chemokine stromal derived factor-1α (SDF-1α) has been implicated recently in the chemotaxis of primitive human hematopoietic cells, suggesting that pluripotent human stem cells express the SDF-1α receptor, CXCR4. By using flow cytometry and confocal microscopy, we have identified and isolated primitive subsets of human CXCR4+ and CXCR4- cells. Distinctions in the progenitor content and response to SDF-1α in vitro indicate that CXCR4+ and CXCR4- cells represent discrete populations of primitive blood cells. The i.v. transplantation of these subfractions into immune-deficient mice established that both possess comparable engraftment capacity in vivo. Human myeloid, lymphoid, and primitive CD34+ CXCR4+ cells were present in chimeric animals transplanted with either subset, indicating that CXCR4+ and CXCR4- stem cells have equivalent proliferative and differentiative abilities. Our study indicates that the human stem cell compartment is heterogeneous for CXCR4 expression, suggesting that the relationship between CXCR4 expression and stem cell repopulating function is not obligatory.
AB - The chemokine stromal derived factor-1α (SDF-1α) has been implicated recently in the chemotaxis of primitive human hematopoietic cells, suggesting that pluripotent human stem cells express the SDF-1α receptor, CXCR4. By using flow cytometry and confocal microscopy, we have identified and isolated primitive subsets of human CXCR4+ and CXCR4- cells. Distinctions in the progenitor content and response to SDF-1α in vitro indicate that CXCR4+ and CXCR4- cells represent discrete populations of primitive blood cells. The i.v. transplantation of these subfractions into immune-deficient mice established that both possess comparable engraftment capacity in vivo. Human myeloid, lymphoid, and primitive CD34+ CXCR4+ cells were present in chimeric animals transplanted with either subset, indicating that CXCR4+ and CXCR4- stem cells have equivalent proliferative and differentiative abilities. Our study indicates that the human stem cell compartment is heterogeneous for CXCR4 expression, suggesting that the relationship between CXCR4 expression and stem cell repopulating function is not obligatory.
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U2 - 10.1073/pnas.97.26.14626
DO - 10.1073/pnas.97.26.14626
M3 - Article
C2 - 11121064
AN - SCOPUS:14344275786
SN - 0027-8424
VL - 97
SP - 14626
EP - 14631
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
ER -