The identification of Pseudomonas aeruginosa persisters using flow cytometry

Shannen Grandy; Renee Raudonis; Zhenyu Cheng

doi:10.1099/mic.0.001252

The identification of Pseudomonas aeruginosa persisters using flow cytometry

Shannen Grandy, Renee Raudonis, Zhenyu Cheng

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Pseudomonas aeruginosa persisters are a rare and poorly characterized subpopulation of cells that are responsible for many recurrent infections. The lack of knowledge on the mechanisms that lead to persister cell development is mainly a result of the difficulty in isolating and characterizing this rare population. Flow cytometry is an ideal method for identifying such subpopulations because it allows for high-content single-cell analysis. However, there are fewer established protocols for bacterial flow cytometry compared to mammalian cell work. Herein, we describe and propose a flow cytometry protocol to identify and isolate P. aeruginosa persister cells. Additionally, we show that the percentage of potential persister cells increases with increasing antibiotic concentrations above the MIC.

Original language	English
Article number	001252
Journal	Microbiology (United Kingdom)
Volume	168
Issue number	10
DOIs	https://doi.org/10.1099/mic.0.001252
Publication status	Published - 2022

Bibliographical note

Funding Information:
This work was supported by a Cystic Fibrosis Canada Martha Morton Early Career Investigator Award to Z.C. S.G. is a recipient of a Nova Scotia Graduate Student Award and a Scotia Scholars Award from Research Nova Scotia.

Publisher Copyright:
© 2022 The Authors.

ASJC Scopus Subject Areas

Microbiology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1099/mic.0.001252

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abstract = "Pseudomonas aeruginosa persisters are a rare and poorly characterized subpopulation of cells that are responsible for many recurrent infections. The lack of knowledge on the mechanisms that lead to persister cell development is mainly a result of the difficulty in isolating and characterizing this rare population. Flow cytometry is an ideal method for identifying such subpopulations because it allows for high-content single-cell analysis. However, there are fewer established protocols for bacterial flow cytometry compared to mammalian cell work. Herein, we describe and propose a flow cytometry protocol to identify and isolate P. aeruginosa persister cells. Additionally, we show that the percentage of potential persister cells increases with increasing antibiotic concentrations above the MIC.",

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The identification of Pseudomonas aeruginosa persisters using flow cytometry

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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