The microglial endocannabinoid system is similarly regulated by lipopolysaccharide and interferon gamma

Alexander P. Young, Eileen M. Denovan-Wright

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Perturbation of the endocannabinoid system can have profound effects on immune function and synaptic plasticity. Microglia are one of few cell types with a self-contained endocannabinoid system and are positioned at the interface between the immune system and the central nervous system. Past work has produced conflicting results with respect to the effects of pro-inflammatory conditions on the microglial endocannabinoid system. Thus, we systematically investigated the relationship between the concentration of two distinct pro-inflammatory stimuli, lipopolysaccharide and interferon gamma, on the abundance of components of the endocannabinoid system within microglia. Here we show that lipopolysaccharide and interferon gamma influence messenger RNA abundances of the microglial endocannabinoid system in a concentration-dependent manner. Furthermore, we demonstrate that the efficacy of different synthetic cannabinoid treatments with respect to inhibition of microglia nitric oxide release is dependent on the concentration and type of pro-inflammatory stimuli presented to the microglia. This indicates that different pro-inflammatory stimuli influence the capacity of microglia to synthesize, degrade, and respond to cannabinoids which has implications for the development of cannabinoid-based treatments for neuroinflammation.

Original languageEnglish
Article number577971
JournalJournal of Neuroimmunology
Volume372
DOIs
Publication statusPublished - Nov 15 2022

Bibliographical note

Funding Information:
The present study was funded by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to E.M.D-W. (grant # RGPIN-2019-37999 ) and an NSERC Alexander Graham Bell Canada Graduate Scholarship (CGS-D) to A.P.Y.

Publisher Copyright:
© 2022 Elsevier B.V.

ASJC Scopus Subject Areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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Young, A. P., & Denovan-Wright, E. M. (2022). The microglial endocannabinoid system is similarly regulated by lipopolysaccharide and interferon gamma. Journal of Neuroimmunology, 372, Article 577971. https://doi.org/10.1016/j.jneuroim.2022.577971