The use of microsatellite markers to study genetic diversity in Indonesian sheep

Jakaria; M. S.A. Zein; S. Sulandari; Subandriyo; Muladno

doi:10.14710/jitaa.37.1.1-9

The use of microsatellite markers to study genetic diversity in Indonesian sheep

Jakaria, M. S.A. Zein, S. Sulandari, Subandriyo, Muladno

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The purpose of this research was to study genetic diversity in Indonesian sheep population using microsatellite markers. A total of 18 microsatellite loci have been used for genotyping Indonesian sheep. Total sheep blood 200 samples were extracted from garut sheep of fighting and meat types, purbalingga sheep, batur sheep and jember sheep populations by using a salting out method. Microsatellite loci data were analyzed using POPGENE 3.2 software. Based on this study obtained 180 alleles from 17 microsatellite loci, while average number of alleles was 6.10 alleles (6 to 18 alleles) from five Indonesian sheep populations (garut sheep of fighting type, garut sheep of meat type, purbalingga sheep, batur sheep and jember sheep population). The average of observed heterozygosity (Ho) and expected heterozygosity (He) values were 0.5749 and 0.6896, respectively, while the genetic differentiation for inbreeding among population (FIS), within population (FIT) and average genetic differentiation (FST) were 0.1006, 0.1647 and 0.0712, respectively. Genetic distance and genetic tree showed that Indonesian sheep population was distinct from garut sheep of fighting and meat types, purbalingga sheep, batur sheep and jember sheep population. Based on this results were needed a strategy for conservation and breeding programs in each Indonesian sheep population.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of the Indonesian Tropical Animal Agriculture
Volume	37
Issue number	1
DOIs	https://doi.org/10.14710/jitaa.37.1.1-9
Publication status	Published - Mar 2012
Externally published	Yes

Bibliographical note

Funding Information:
Authors thank to IAEA (International Atomic Energy Agency) for funding this research (contract number: CRP D.3.10.25). Authors also thank to Prof. Han Jianlin for genotyping analysis in Laboratory of Livestock and Forage Genetic Resources, CAAS-ILRI JLLFGR Beijing, China. Finally, authors thank to Eryk Andreas, MSc for assisting in data analysis.

Publisher Copyright:
© 2012 Diponegoro University. All rights reserved.

ASJC Scopus Subject Areas

Animal Science and Zoology
General Veterinary

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10.14710/jitaa.37.1.1-9

Cite this

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title = "The use of microsatellite markers to study genetic diversity in Indonesian sheep",

abstract = "The purpose of this research was to study genetic diversity in Indonesian sheep population using microsatellite markers. A total of 18 microsatellite loci have been used for genotyping Indonesian sheep. Total sheep blood 200 samples were extracted from garut sheep of fighting and meat types, purbalingga sheep, batur sheep and jember sheep populations by using a salting out method. Microsatellite loci data were analyzed using POPGENE 3.2 software. Based on this study obtained 180 alleles from 17 microsatellite loci, while average number of alleles was 6.10 alleles (6 to 18 alleles) from five Indonesian sheep populations (garut sheep of fighting type, garut sheep of meat type, purbalingga sheep, batur sheep and jember sheep population). The average of observed heterozygosity (Ho) and expected heterozygosity (He) values were 0.5749 and 0.6896, respectively, while the genetic differentiation for inbreeding among population (FIS), within population (FIT) and average genetic differentiation (FST) were 0.1006, 0.1647 and 0.0712, respectively. Genetic distance and genetic tree showed that Indonesian sheep population was distinct from garut sheep of fighting and meat types, purbalingga sheep, batur sheep and jember sheep population. Based on this results were needed a strategy for conservation and breeding programs in each Indonesian sheep population.",

author = "Jakaria and Zein, {M. S.A.} and S. Sulandari and Subandriyo and Muladno",

note = "Funding Information: Authors thank to IAEA (International Atomic Energy Agency) for funding this research (contract number: CRP D.3.10.25). Authors also thank to Prof. Han Jianlin for genotyping analysis in Laboratory of Livestock and Forage Genetic Resources, CAAS-ILRI JLLFGR Beijing, China. Finally, authors thank to Eryk Andreas, MSc for assisting in data analysis. Publisher Copyright: {\textcopyright} 2012 Diponegoro University. All rights reserved.",

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The use of microsatellite markers to study genetic diversity in Indonesian sheep

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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