TPC proteins are phosphoinositide- Activated sodium-selective ion channels in endosomes and lysosomes

Xiang Wang; Xiaoli Zhang; Xian Ping Dong; Mohammad Samie; Xinran Li; Xiping Cheng; Andrew Goschka; Dongbiao Shen; Yandong Zhou; Janice Harlow; Michael X. Zhu; David E. Clapham; Dejian Ren; Haoxing Xu

doi:10.1016/j.cell.2012.08.036

TPC proteins are phosphoinositide- Activated sodium-selective ion channels in endosomes and lysosomes

Xiang Wang, Xiaoli Zhang, Xian Ping Dong, Mohammad Samie, Xinran Li, Xiping Cheng, Andrew Goschka, Dongbiao Shen, Yandong Zhou, Janice Harlow, Michael X. Zhu, David E. Clapham, Dejian Ren, Haoxing Xu

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Abstract

Mammalian two-pore channel proteins (TPC1, TPC2; TPCN1, TPCN2) encode ion channels in intracellular endosomes and lysosomes and were proposed to mediate endolysosomal calcium release triggered by the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). By directly recording TPCs in endolysosomes from wild-type and TPC double-knockout mice, here we show that, in contrast to previous conclusions, TPCs are in fact sodium-selective channels activated by PI(3,5)P₂ and are not activated by NAADP. Moreover, the primary endolysosomal ion is Na⁺, not K⁺, as had been previously assumed. These findings suggest that the organellar membrane potential may undergo large regulatory changes and may explain the specificity of PI(3,5)P₂ in regulating the fusogenic potential of intracellular organelles.

Original language	English
Pages (from-to)	372-383
Number of pages	12
Journal	Cell
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/j.cell.2012.08.036
Publication status	Published - Oct 12 2012
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by NIH R01 grants (NS062792 to H.X., NS055293 to D.R., GM081658 to M.X.Z., and HHMI to D.E.C.) and a Sloan Research Fellowship (to H.X.). We thank the Gene Manipulation Facility of Children's Hospital Boston (TPC1) and the Transgenic and Chimera Mouse Facility at the University of Pennsylvania (TPC2) for embryonic stem cell injection. The authors are listed in the order of their institutions; X.W. initiated the project; X.W., X.Z., and X.-p.D. performed the electrophysiology experiments; X.W. also performed the Ca 2+ imaging experiments; M.S. and A.G. performed the ionic composition experiments; X.L., X.C., D.S., and M.X.Z. contributed the reagents; D.R. and J.H. developed the TPC1, TPC2 knockout mouse lines and the hTPC1, hTPC2, hTPC1ΔN, and hTPC2ΔN constructs; Y.Z. measured the glucose- and NAADP-induced Ca 2+ responses in the TPC mutant islets compared with wild-type; development of the TPC1 knockout was carried out by D.R. in the laboratory of D.E.C.; and H.X. and D.E.C. wrote the paper with input from all authors. We are grateful to Drs. Susan Slaugenhaupt and James Slama for sharing reagents; Drs. Ted Huston, Hollis Showalter, Yafei Jin, Leslie Satin, Jianhua Ren, Peter Arvan, and Gautam Rajpal for assistance; and Dr. Richard Hume for comments on an earlier version of the manuscript. We appreciate the encouragement and helpful comments from other members of the Xu, Ren, and Clapham laboratories.

ASJC Scopus Subject Areas

General Biochemistry,Genetics and Molecular Biology

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@article{2d65a7bed53b42028213c0010a0525c2,

title = "TPC proteins are phosphoinositide- Activated sodium-selective ion channels in endosomes and lysosomes",

abstract = "Mammalian two-pore channel proteins (TPC1, TPC2; TPCN1, TPCN2) encode ion channels in intracellular endosomes and lysosomes and were proposed to mediate endolysosomal calcium release triggered by the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). By directly recording TPCs in endolysosomes from wild-type and TPC double-knockout mice, here we show that, in contrast to previous conclusions, TPCs are in fact sodium-selective channels activated by PI(3,5)P2 and are not activated by NAADP. Moreover, the primary endolysosomal ion is Na+, not K+, as had been previously assumed. These findings suggest that the organellar membrane potential may undergo large regulatory changes and may explain the specificity of PI(3,5)P2 in regulating the fusogenic potential of intracellular organelles.",

author = "Xiang Wang and Xiaoli Zhang and Dong, {Xian Ping} and Mohammad Samie and Xinran Li and Xiping Cheng and Andrew Goschka and Dongbiao Shen and Yandong Zhou and Janice Harlow and Zhu, {Michael X.} and Clapham, {David E.} and Dejian Ren and Haoxing Xu",

note = "Funding Information: This work was supported by NIH R01 grants (NS062792 to H.X., NS055293 to D.R., GM081658 to M.X.Z., and HHMI to D.E.C.) and a Sloan Research Fellowship (to H.X.). We thank the Gene Manipulation Facility of Children's Hospital Boston (TPC1) and the Transgenic and Chimera Mouse Facility at the University of Pennsylvania (TPC2) for embryonic stem cell injection. The authors are listed in the order of their institutions; X.W. initiated the project; X.W., X.Z., and X.-p.D. performed the electrophysiology experiments; X.W. also performed the Ca 2+ imaging experiments; M.S. and A.G. performed the ionic composition experiments; X.L., X.C., D.S., and M.X.Z. contributed the reagents; D.R. and J.H. developed the TPC1, TPC2 knockout mouse lines and the hTPC1, hTPC2, hTPC1ΔN, and hTPC2ΔN constructs; Y.Z. measured the glucose- and NAADP-induced Ca 2+ responses in the TPC mutant islets compared with wild-type; development of the TPC1 knockout was carried out by D.R. in the laboratory of D.E.C.; and H.X. and D.E.C. wrote the paper with input from all authors. We are grateful to Drs. Susan Slaugenhaupt and James Slama for sharing reagents; Drs. Ted Huston, Hollis Showalter, Yafei Jin, Leslie Satin, Jianhua Ren, Peter Arvan, and Gautam Rajpal for assistance; and Dr. Richard Hume for comments on an earlier version of the manuscript. We appreciate the encouragement and helpful comments from other members of the Xu, Ren, and Clapham laboratories. ",

year = "2012",

month = oct,

day = "12",

doi = "10.1016/j.cell.2012.08.036",

language = "English",

volume = "151",

pages = "372--383",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

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TY - JOUR

T1 - TPC proteins are phosphoinositide- Activated sodium-selective ion channels in endosomes and lysosomes

AU - Wang, Xiang

AU - Zhang, Xiaoli

AU - Dong, Xian Ping

AU - Samie, Mohammad

AU - Li, Xinran

AU - Cheng, Xiping

AU - Goschka, Andrew

AU - Shen, Dongbiao

AU - Zhou, Yandong

AU - Harlow, Janice

AU - Zhu, Michael X.

AU - Clapham, David E.

AU - Ren, Dejian

AU - Xu, Haoxing

N1 - Funding Information: This work was supported by NIH R01 grants (NS062792 to H.X., NS055293 to D.R., GM081658 to M.X.Z., and HHMI to D.E.C.) and a Sloan Research Fellowship (to H.X.). We thank the Gene Manipulation Facility of Children's Hospital Boston (TPC1) and the Transgenic and Chimera Mouse Facility at the University of Pennsylvania (TPC2) for embryonic stem cell injection. The authors are listed in the order of their institutions; X.W. initiated the project; X.W., X.Z., and X.-p.D. performed the electrophysiology experiments; X.W. also performed the Ca 2+ imaging experiments; M.S. and A.G. performed the ionic composition experiments; X.L., X.C., D.S., and M.X.Z. contributed the reagents; D.R. and J.H. developed the TPC1, TPC2 knockout mouse lines and the hTPC1, hTPC2, hTPC1ΔN, and hTPC2ΔN constructs; Y.Z. measured the glucose- and NAADP-induced Ca 2+ responses in the TPC mutant islets compared with wild-type; development of the TPC1 knockout was carried out by D.R. in the laboratory of D.E.C.; and H.X. and D.E.C. wrote the paper with input from all authors. We are grateful to Drs. Susan Slaugenhaupt and James Slama for sharing reagents; Drs. Ted Huston, Hollis Showalter, Yafei Jin, Leslie Satin, Jianhua Ren, Peter Arvan, and Gautam Rajpal for assistance; and Dr. Richard Hume for comments on an earlier version of the manuscript. We appreciate the encouragement and helpful comments from other members of the Xu, Ren, and Clapham laboratories.

PY - 2012/10/12

Y1 - 2012/10/12

N2 - Mammalian two-pore channel proteins (TPC1, TPC2; TPCN1, TPCN2) encode ion channels in intracellular endosomes and lysosomes and were proposed to mediate endolysosomal calcium release triggered by the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). By directly recording TPCs in endolysosomes from wild-type and TPC double-knockout mice, here we show that, in contrast to previous conclusions, TPCs are in fact sodium-selective channels activated by PI(3,5)P2 and are not activated by NAADP. Moreover, the primary endolysosomal ion is Na+, not K+, as had been previously assumed. These findings suggest that the organellar membrane potential may undergo large regulatory changes and may explain the specificity of PI(3,5)P2 in regulating the fusogenic potential of intracellular organelles.

AB - Mammalian two-pore channel proteins (TPC1, TPC2; TPCN1, TPCN2) encode ion channels in intracellular endosomes and lysosomes and were proposed to mediate endolysosomal calcium release triggered by the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). By directly recording TPCs in endolysosomes from wild-type and TPC double-knockout mice, here we show that, in contrast to previous conclusions, TPCs are in fact sodium-selective channels activated by PI(3,5)P2 and are not activated by NAADP. Moreover, the primary endolysosomal ion is Na+, not K+, as had been previously assumed. These findings suggest that the organellar membrane potential may undergo large regulatory changes and may explain the specificity of PI(3,5)P2 in regulating the fusogenic potential of intracellular organelles.

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TPC proteins are phosphoinositide- Activated sodium-selective ion channels in endosomes and lysosomes

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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