Trans-10,cis-12-conjugated linoleic acid worsens renal pathology and alters cyclooxygenase derived oxylipins in obesity-associated nephropathy

Yang Zhan; Hong Shi; Stephanie P.B. Caligiuri; Yinghong Wu; Vanessa Declercq; Carla G. Taylor; Peter Zahradka; Malcolm R. Ogborn; Harold M. Aukema

doi:10.1016/j.jnutbio.2014.09.011

Trans-10,cis-12-conjugated linoleic acid worsens renal pathology and alters cyclooxygenase derived oxylipins in obesity-associated nephropathy

Yang Zhan, Hong Shi, Stephanie P.B. Caligiuri, Yinghong Wu, Vanessa Declercq, Carla G. Taylor, Peter Zahradka, Malcolm R. Ogborn, Harold M. Aukema

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Dietary conjugated linoleic acid (CLA) reduces indicators of early renal disease progression and the associated elevated cyclooxygenase (COX) levels in young obese rats with obesity-associated nephropathy (OAN). Therefore, renal function and injury and COX and its metabolites were assessed in obese fa/fa Zucker rats with more advanced renal disease. Obese rats at 16weeks of age were provided with either cis(c)9, trans(t)11 (fa/fa-9,11) or t10,c12 (fa/fa-10,12) CLA for 8weeks, and compared to lean (lean-CTL) and obese (fa/fa-CTL) rats provided the control diet without CLA. Obese rats displayed significantly reduced renal function and increased renal injury compared to lean rats. In the obese rat groups, glomerular hypertrophy was reduced in both CLA-supplemented groups. While all other measures of renal function or injury were not different in fa/fa-9,11 compared to fa/fa-CTL rats, the fa/fa-10,12 rats had greater renal hypertrophy, glomerular fibrosis, fibrosis, tubular casts and macrophage infiltration compared to the fa/fa-CTL and fa/fa-9,11 groups. The fa/fa-10,12 group also had elevated levels of renal COX1, which was associated with increased levels of two oxylipins produced by this enzyme, 6-keto-prostaglandin F_1α, and thromboxane B₂. Renal linoleic acid and its lipoxygenase products also were lower in obese compared to lean rats, but CLA supplementation had no effect on these or any other lipoxygenase oxylipins. In summary, supplementation with c9,t11 CLA did not improve more advanced OAN and t10,c12 CLA worsened the renal pathology. Altered production of select COX1 derived oxylipins was associated with the detrimental effect of the t10,c12 isomer.

Original language	English
Pages (from-to)	130-137
Number of pages	8
Journal	Journal of Nutritional Biochemistry
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/j.jnutbio.2014.09.011
Publication status	Published - Feb 1 2015
Externally published	Yes

Bibliographical note

Funding Information:
Grants, sponsors and funding sources: This work was supported by grant funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery program (H.M.A.) , Dairy Farmers of Canada and an NSERC Collaborative Research Development Project (C.G.T. and P.Z.), and NSERC graduate (V.D.) and undergraduate (Y.W.) scholarships.

Publisher Copyright:
© 2015 Elsevier Inc.

ASJC Scopus Subject Areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology
Nutrition and Dietetics
Clinical Biochemistry

PubMed: MeSH publication types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jnutbio.2014.09.011

Cite this

Zhan, Y., Shi, H., Caligiuri, S. P. B., Wu, Y., Declercq, V., Taylor, C. G., Zahradka, P., Ogborn, M. R., & Aukema, H. M. (2015). Trans-10,cis-12-conjugated linoleic acid worsens renal pathology and alters cyclooxygenase derived oxylipins in obesity-associated nephropathy. Journal of Nutritional Biochemistry, 26(2), 130-137. https://doi.org/10.1016/j.jnutbio.2014.09.011

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title = "Trans-10,cis-12-conjugated linoleic acid worsens renal pathology and alters cyclooxygenase derived oxylipins in obesity-associated nephropathy",

abstract = "Dietary conjugated linoleic acid (CLA) reduces indicators of early renal disease progression and the associated elevated cyclooxygenase (COX) levels in young obese rats with obesity-associated nephropathy (OAN). Therefore, renal function and injury and COX and its metabolites were assessed in obese fa/fa Zucker rats with more advanced renal disease. Obese rats at 16weeks of age were provided with either cis(c)9, trans(t)11 (fa/fa-9,11) or t10,c12 (fa/fa-10,12) CLA for 8weeks, and compared to lean (lean-CTL) and obese (fa/fa-CTL) rats provided the control diet without CLA. Obese rats displayed significantly reduced renal function and increased renal injury compared to lean rats. In the obese rat groups, glomerular hypertrophy was reduced in both CLA-supplemented groups. While all other measures of renal function or injury were not different in fa/fa-9,11 compared to fa/fa-CTL rats, the fa/fa-10,12 rats had greater renal hypertrophy, glomerular fibrosis, fibrosis, tubular casts and macrophage infiltration compared to the fa/fa-CTL and fa/fa-9,11 groups. The fa/fa-10,12 group also had elevated levels of renal COX1, which was associated with increased levels of two oxylipins produced by this enzyme, 6-keto-prostaglandin F1α, and thromboxane B2. Renal linoleic acid and its lipoxygenase products also were lower in obese compared to lean rats, but CLA supplementation had no effect on these or any other lipoxygenase oxylipins. In summary, supplementation with c9,t11 CLA did not improve more advanced OAN and t10,c12 CLA worsened the renal pathology. Altered production of select COX1 derived oxylipins was associated with the detrimental effect of the t10,c12 isomer.",

author = "Yang Zhan and Hong Shi and Caligiuri, {Stephanie P.B.} and Yinghong Wu and Vanessa Declercq and Taylor, {Carla G.} and Peter Zahradka and Ogborn, {Malcolm R.} and Aukema, {Harold M.}",

note = "Funding Information: Grants, sponsors and funding sources: This work was supported by grant funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery program (H.M.A.) , Dairy Farmers of Canada and an NSERC Collaborative Research Development Project (C.G.T. and P.Z.), and NSERC graduate (V.D.) and undergraduate (Y.W.) scholarships. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

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T1 - Trans-10,cis-12-conjugated linoleic acid worsens renal pathology and alters cyclooxygenase derived oxylipins in obesity-associated nephropathy

AU - Zhan, Yang

AU - Shi, Hong

AU - Caligiuri, Stephanie P.B.

AU - Wu, Yinghong

AU - Declercq, Vanessa

AU - Taylor, Carla G.

AU - Zahradka, Peter

AU - Ogborn, Malcolm R.

AU - Aukema, Harold M.

N1 - Funding Information: Grants, sponsors and funding sources: This work was supported by grant funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery program (H.M.A.) , Dairy Farmers of Canada and an NSERC Collaborative Research Development Project (C.G.T. and P.Z.), and NSERC graduate (V.D.) and undergraduate (Y.W.) scholarships. Publisher Copyright: © 2015 Elsevier Inc.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Dietary conjugated linoleic acid (CLA) reduces indicators of early renal disease progression and the associated elevated cyclooxygenase (COX) levels in young obese rats with obesity-associated nephropathy (OAN). Therefore, renal function and injury and COX and its metabolites were assessed in obese fa/fa Zucker rats with more advanced renal disease. Obese rats at 16weeks of age were provided with either cis(c)9, trans(t)11 (fa/fa-9,11) or t10,c12 (fa/fa-10,12) CLA for 8weeks, and compared to lean (lean-CTL) and obese (fa/fa-CTL) rats provided the control diet without CLA. Obese rats displayed significantly reduced renal function and increased renal injury compared to lean rats. In the obese rat groups, glomerular hypertrophy was reduced in both CLA-supplemented groups. While all other measures of renal function or injury were not different in fa/fa-9,11 compared to fa/fa-CTL rats, the fa/fa-10,12 rats had greater renal hypertrophy, glomerular fibrosis, fibrosis, tubular casts and macrophage infiltration compared to the fa/fa-CTL and fa/fa-9,11 groups. The fa/fa-10,12 group also had elevated levels of renal COX1, which was associated with increased levels of two oxylipins produced by this enzyme, 6-keto-prostaglandin F1α, and thromboxane B2. Renal linoleic acid and its lipoxygenase products also were lower in obese compared to lean rats, but CLA supplementation had no effect on these or any other lipoxygenase oxylipins. In summary, supplementation with c9,t11 CLA did not improve more advanced OAN and t10,c12 CLA worsened the renal pathology. Altered production of select COX1 derived oxylipins was associated with the detrimental effect of the t10,c12 isomer.

AB - Dietary conjugated linoleic acid (CLA) reduces indicators of early renal disease progression and the associated elevated cyclooxygenase (COX) levels in young obese rats with obesity-associated nephropathy (OAN). Therefore, renal function and injury and COX and its metabolites were assessed in obese fa/fa Zucker rats with more advanced renal disease. Obese rats at 16weeks of age were provided with either cis(c)9, trans(t)11 (fa/fa-9,11) or t10,c12 (fa/fa-10,12) CLA for 8weeks, and compared to lean (lean-CTL) and obese (fa/fa-CTL) rats provided the control diet without CLA. Obese rats displayed significantly reduced renal function and increased renal injury compared to lean rats. In the obese rat groups, glomerular hypertrophy was reduced in both CLA-supplemented groups. While all other measures of renal function or injury were not different in fa/fa-9,11 compared to fa/fa-CTL rats, the fa/fa-10,12 rats had greater renal hypertrophy, glomerular fibrosis, fibrosis, tubular casts and macrophage infiltration compared to the fa/fa-CTL and fa/fa-9,11 groups. The fa/fa-10,12 group also had elevated levels of renal COX1, which was associated with increased levels of two oxylipins produced by this enzyme, 6-keto-prostaglandin F1α, and thromboxane B2. Renal linoleic acid and its lipoxygenase products also were lower in obese compared to lean rats, but CLA supplementation had no effect on these or any other lipoxygenase oxylipins. In summary, supplementation with c9,t11 CLA did not improve more advanced OAN and t10,c12 CLA worsened the renal pathology. Altered production of select COX1 derived oxylipins was associated with the detrimental effect of the t10,c12 isomer.

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Trans-10,cis-12-conjugated linoleic acid worsens renal pathology and alters cyclooxygenase derived oxylipins in obesity-associated nephropathy

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

UN SDGs

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