Abstract
A plethora of growth factors regulate keratinocyte proliferation and differentiation that control hair morphogenesis and skin barrier formation. Wavy hair phenotypes in mice result from naturally occurring loss-of-function mutations in the genes for TGF-α and EGFR. Conversely, excessive activities of TGF-α/EGFR result in hairless phenotypes and skin cancers. Unexpectedly, we found that mice lacking the Trpv3 gene also exhibit wavy hair coat and curly whiskers. Here we show that keratinocyte TRPV3, a member of the transient receptor potential (TRP) family of Ca2+-permeant channels, forms a signaling complex with TGF-α/EGFR. Activation of EGFR leads to increased TRPV3 channel activity, which in turn stimulates TGF-α release. TRPV3 is also required for the formation of the skin barrier by regulating the activities of transglutaminases, a family of Ca2+-dependent crosslinking enzymes essential for keratinocyte cornification. Our results show that a TRP channel plays a role in regulating growth factor signaling by direct complex formation.
Original language | English |
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Pages (from-to) | 331-343 |
Number of pages | 13 |
Journal | Cell |
Volume | 141 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 2010 |
Externally published | Yes |
Bibliographical note
Funding Information:This work is supported by HHMI (to N.C.A and D.E.C), startup funds to H.X. from the Department of MCDB and Biological Science Scholar Program, and the University of Michigan and NIH RO1 grants (NS062792 to H.X. and AR045973 to A.A.D.). We thank Dr. Leonidas Tsiokas for the EGFR construct, Dr. Nan Hatch and Dr. Dave Ornitz for the FGFR2 constructs, and Dr. Makato Suzuki for TRPV4 KO mice. We are grateful to Y. Fujiwara (Division of Hematology Transgenic core facility), S. Hein, X. Wang (Rockefeller University), X. Wang, E. Mills, R. Hume, J. Kuwada, M. Akaaboune, and C. Collins for assistance and L. Yue and D. Ren for comments on an earlier version of the manuscript. We appreciate the encouragement and helpful comments from other members of the Xu and Clapham laboratories. D.E.C. is founder of Hydra, which is currently working on TRPV3 antagonists.
ASJC Scopus Subject Areas
- General Biochemistry,Genetics and Molecular Biology