Up-regulation of estrogen receptor mRNA and estrogen receptor activity by estradiol in liver of rainbow trout and other teleostean fish

M. E. MacKay, J. Raelson, C. B. Lazier

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

Injection of estradiol (E2) into immature rainbow trout resulted in the induction of the hepatic vitellogenin gene mediated by the nuclear estrogen receptor (ER). Liver ER mRNA rose markedly on E2 treatment in three groups of trout kept at different temperatures. Only in the group kept at 4°C did the total cellular ER, as measured by [3H]estradiol-binding activity in nuclear and cytosol fractions, parallel the ER mRNA level. In fish kept at 9°C and 15°C, the ratio of total ER activity to ER mRNA fell during chronic E2 treatment, probably reflecting translational or post-translational control mechanisms. Upregulation of ER mRNA also occurred in sea raven, sculpin, winter flounder, and Atlantic salmon after E2 treatment. Intrahepatic ER activity rose proportionately in Atlantic salmon kept at 6-9°C but not in sea raven, sculpin, or flounder. We conclude that the regulation of ER expression in teleosts is complex and includes transcriptional, translational, and posttranslational elements and is influenced by environmental temperature.

Original languageEnglish
Pages (from-to)201-209
Number of pages9
JournalComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume115
Issue number3
DOIs
Publication statusPublished - Nov 1996

Bibliographical note

Funding Information:
Grant supportw as from the Medical Research Council of Can&. We

ASJC Scopus Subject Areas

  • Immunology
  • Pharmacology

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