Zebrafish mast cells possess an Fce{open}RI-like receptor and participate in innate and adaptive immune responses

Sahar Da'as; Evelyn M. Teh; J. Tristan Dobson; Gheyath K. Nasrallah; Eileen R. McBride; Hao Wang; Donna S. Neuberg; Jean S. Marshall; Tong Jun Lin; Jason N. Berman

doi:10.1016/j.dci.2010.09.001

Zebrafish mast cells possess an Fce{open}RI-like receptor and participate in innate and adaptive immune responses

Sahar Da'as, Evelyn M. Teh, J. Tristan Dobson, Gheyath K. Nasrallah, Eileen R. McBride, Hao Wang, Donna S. Neuberg, Jean S. Marshall, Tong Jun Lin, Jason N. Berman

Medicine

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p= 0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p= 0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (Fce{open}RIγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish Fce{open}RI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p= 0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.

Original language	English
Pages (from-to)	125-134
Number of pages	10
Journal	Developmental and Comparative Immunology
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/j.dci.2010.09.001
Publication status	Published - Jan 2011

Bibliographical note

Funding Information:
We would like to thank Chris Hall and Phil Crosier for providing myd88::GFP embryos, Jeffrey Yoder for providing fcer1g and fcer1gl cDNA clones and critical review of the manuscript. We would like to thank David Traver for critical review of the manuscript. We would like to thank Graham Dellaire for assistance with reviewing gene conservation including assisting with generating phylogenetic trees. Western blots were conducted in the laboratory of Rafael Garduno. We would like to thank Jocelyn Jaques for administrative assistance. J.N.B. is supported by Dalhousie Clinical Scholar and CIHR-NSHRF Regional Partnership Awards.

ASJC Scopus Subject Areas

Immunology
Developmental Biology

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10.1016/j.dci.2010.09.001

Cite this

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title = "Zebrafish mast cells possess an Fce{open}RI-like receptor and participate in innate and adaptive immune responses",

abstract = "We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p= 0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p= 0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (Fce{open}RIγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish Fce{open}RI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p= 0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.",

author = "Sahar Da'as and Teh, {Evelyn M.} and Dobson, {J. Tristan} and Nasrallah, {Gheyath K.} and McBride, {Eileen R.} and Hao Wang and Neuberg, {Donna S.} and Marshall, {Jean S.} and Lin, {Tong Jun} and Berman, {Jason N.}",

note = "Funding Information: We would like to thank Chris Hall and Phil Crosier for providing myd88::GFP embryos, Jeffrey Yoder for providing fcer1g and fcer1gl cDNA clones and critical review of the manuscript. We would like to thank David Traver for critical review of the manuscript. We would like to thank Graham Dellaire for assistance with reviewing gene conservation including assisting with generating phylogenetic trees. Western blots were conducted in the laboratory of Rafael Garduno. We would like to thank Jocelyn Jaques for administrative assistance. J.N.B. is supported by Dalhousie Clinical Scholar and CIHR-NSHRF Regional Partnership Awards. ",

year = "2011",

month = jan,

doi = "10.1016/j.dci.2010.09.001",

language = "English",

volume = "35",

pages = "125--134",

journal = "Developmental and Comparative Immunology",

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T1 - Zebrafish mast cells possess an Fce{open}RI-like receptor and participate in innate and adaptive immune responses

AU - Da'as, Sahar

AU - Teh, Evelyn M.

AU - Dobson, J. Tristan

AU - Nasrallah, Gheyath K.

AU - McBride, Eileen R.

AU - Wang, Hao

AU - Neuberg, Donna S.

AU - Marshall, Jean S.

AU - Lin, Tong Jun

AU - Berman, Jason N.

N1 - Funding Information: We would like to thank Chris Hall and Phil Crosier for providing myd88::GFP embryos, Jeffrey Yoder for providing fcer1g and fcer1gl cDNA clones and critical review of the manuscript. We would like to thank David Traver for critical review of the manuscript. We would like to thank Graham Dellaire for assistance with reviewing gene conservation including assisting with generating phylogenetic trees. Western blots were conducted in the laboratory of Rafael Garduno. We would like to thank Jocelyn Jaques for administrative assistance. J.N.B. is supported by Dalhousie Clinical Scholar and CIHR-NSHRF Regional Partnership Awards.

PY - 2011/1

Y1 - 2011/1

N2 - We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p= 0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p= 0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (Fce{open}RIγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish Fce{open}RI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p= 0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.

AB - We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p= 0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p= 0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (Fce{open}RIγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish Fce{open}RI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p= 0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.

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SN - 0145-305X

VL - 35

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EP - 134

JO - Developmental and Comparative Immunology

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ER -

Zebrafish mast cells possess an Fce{open}RI-like receptor and participate in innate and adaptive immune responses

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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