A diet-change modulates the previously established bacterial gut community in juvenile brown trout (Salmo trutta)

Stéphanie C. Michl; Matt Beyer; Jenni Marie Ratten; Mario Hasler; Julie LaRoche; Carsten Schulz

doi:10.1038/s41598-019-38800-7

A diet-change modulates the previously established bacterial gut community in juvenile brown trout (Salmo trutta)

Stéphanie C. Michl, Matt Beyer, Jenni Marie Ratten, Mario Hasler, Julie LaRoche, Carsten Schulz

Medicine

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35 Citas (Scopus)

Resumen

The aim of the present study was to investigate the impact of dietary plant proteins on the gut microbiome of first feeding brown trout (Salmo trutta) reproduced from wild stocks and to evaluate whether the initial microbiome of brown trout fry can be permanently manipulated by the first feeding diet. Therefore, brown trout fry was fed diets based on either 0%, 50% or 90% plant-derived proteins from first feeding onwards and via 16S rRNA gene sequencing a strong dietary influence on the bacterial gut community on phylum and order level was detected. Proteobacteria and Fusobacteria were significantly enhanced when fishmeal was integrated into the experimental diet, whereas plant-derived proteins significantly promoted Firmicutes and Bacteroidetes. In order to evaluate whether the first feeding diet had a permanent effect on the initially established microbial gut community of juvenile brown trout, a cross-over diet-change was applied 61 days post first feeding. 48 days after the diet-change, the gut microbiome of all dietary groups was significantly different from the one initially established after first feeding. Moreover, the first feeding diet had no statistically significant influence on the gut microbiome after the diet-change, demonstrating no permanent effect on the gut microbiome formation.

Idioma original	English
Número de artículo	2339
Publicación	Scientific Reports
Volumen	9
N.º	1
DOI	https://doi.org/10.1038/s41598-019-38800-7
Estado	Published - dic. 1 2019

Nota bibliográfica

Funding Information:
We want to express our gratitude to the Skretting Aquaculture Research Center (ARC), Stavanger, Norway, for providing the experimental diets and the chemical analysis of dietary fatty acids and amino acids. There is no conflict of interests. Furthermore, we would like to thank Albrecht Hahn (Fischbrutanstalt Altmühlendorf, Germany) for providing the brown trout eggs and hatching troughs used in this study. We further would like to thank Jennifer Tolman for her assistance in the lab, Jackie Zorz and Dhwani Desai for their help with QIIME data processing, André Comeau for performing the sequencing and his advice on sample processing, and our colleagues from GMA for their support during sampling. We acknowledge financial support by Land Schleswig-Holstein within the funding program Open Access Publikationsfonds. S.C.M. was supported by the Helmholtz Graduate School for Ocean System Science and Technology (HOSST); Geomar Helmholtz Centre for Ocean Research Kiel; http://www.geomar.de/studieren/phd/hosst/. J.M.R. was supported by the NSERC CREATE Transatlantic Ocean System Science and Technology (TOSST); Dalhousie University; http://www.tosst. org/; NSERC DISCOVERY grant to J.L.R. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2019, The Author(s).

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Journal Article
Research Support, Non-U.S. Gov't

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title = "A diet-change modulates the previously established bacterial gut community in juvenile brown trout (Salmo trutta)",

abstract = "The aim of the present study was to investigate the impact of dietary plant proteins on the gut microbiome of first feeding brown trout (Salmo trutta) reproduced from wild stocks and to evaluate whether the initial microbiome of brown trout fry can be permanently manipulated by the first feeding diet. Therefore, brown trout fry was fed diets based on either 0%, 50% or 90% plant-derived proteins from first feeding onwards and via 16S rRNA gene sequencing a strong dietary influence on the bacterial gut community on phylum and order level was detected. Proteobacteria and Fusobacteria were significantly enhanced when fishmeal was integrated into the experimental diet, whereas plant-derived proteins significantly promoted Firmicutes and Bacteroidetes. In order to evaluate whether the first feeding diet had a permanent effect on the initially established microbial gut community of juvenile brown trout, a cross-over diet-change was applied 61 days post first feeding. 48 days after the diet-change, the gut microbiome of all dietary groups was significantly different from the one initially established after first feeding. Moreover, the first feeding diet had no statistically significant influence on the gut microbiome after the diet-change, demonstrating no permanent effect on the gut microbiome formation.",

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note = "Funding Information: We want to express our gratitude to the Skretting Aquaculture Research Center (ARC), Stavanger, Norway, for providing the experimental diets and the chemical analysis of dietary fatty acids and amino acids. There is no conflict of interests. Furthermore, we would like to thank Albrecht Hahn (Fischbrutanstalt Altm{\"u}hlendorf, Germany) for providing the brown trout eggs and hatching troughs used in this study. We further would like to thank Jennifer Tolman for her assistance in the lab, Jackie Zorz and Dhwani Desai for their help with QIIME data processing, Andr{\'e} Comeau for performing the sequencing and his advice on sample processing, and our colleagues from GMA for their support during sampling. We acknowledge financial support by Land Schleswig-Holstein within the funding program Open Access Publikationsfonds. S.C.M. was supported by the Helmholtz Graduate School for Ocean System Science and Technology (HOSST); Geomar Helmholtz Centre for Ocean Research Kiel; http://www.geomar.de/studieren/phd/hosst/. J.M.R. was supported by the NSERC CREATE Transatlantic Ocean System Science and Technology (TOSST); Dalhousie University; http://www.tosst. org/; NSERC DISCOVERY grant to J.L.R. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2019, The Author(s).",

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AU - Hasler, Mario

AU - LaRoche, Julie

AU - Schulz, Carsten

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N2 - The aim of the present study was to investigate the impact of dietary plant proteins on the gut microbiome of first feeding brown trout (Salmo trutta) reproduced from wild stocks and to evaluate whether the initial microbiome of brown trout fry can be permanently manipulated by the first feeding diet. Therefore, brown trout fry was fed diets based on either 0%, 50% or 90% plant-derived proteins from first feeding onwards and via 16S rRNA gene sequencing a strong dietary influence on the bacterial gut community on phylum and order level was detected. Proteobacteria and Fusobacteria were significantly enhanced when fishmeal was integrated into the experimental diet, whereas plant-derived proteins significantly promoted Firmicutes and Bacteroidetes. In order to evaluate whether the first feeding diet had a permanent effect on the initially established microbial gut community of juvenile brown trout, a cross-over diet-change was applied 61 days post first feeding. 48 days after the diet-change, the gut microbiome of all dietary groups was significantly different from the one initially established after first feeding. Moreover, the first feeding diet had no statistically significant influence on the gut microbiome after the diet-change, demonstrating no permanent effect on the gut microbiome formation.

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A diet-change modulates the previously established bacterial gut community in juvenile brown trout (Salmo trutta)

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ASJC Scopus Subject Areas

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