TY - JOUR
T1 - A LIGHT‐SCATTERING TECHNIQUE FOR THE STUDY OF THE PERMEABILITY OF RAT BRAIN SYNAPTOSOMES IN VITRO
AU - Keen, P.
AU - White, T. D.
PY - 1970/5
Y1 - 1970/5
N2 - Abstract— (1) Swelling of synaptosomes was measured spectrophotometrically by recording changes in extinction at 520 nm. (2) Synaptosomes behaved as osmometers in NaCl solutions. When the tonicity of the medium was changed, synaptosome volume changed in accordance with Boyle and van't Hoff's Law. These changes were reversed on restoring the tonicity of the medium. (3) The rate at which a solute entered the synaptosome was determined from the rate of swelling in the presence of that solute. Permeability of synaptosomes to non‐electrolytes was in the order glucose ≪ glycerol < thiourea = formamide < propylene glycol = dimethylsulphoxide. (4) Synaptosomes were freely permeable to ammonium and acetate ions and impermeable to Ca2+, Mg2+, PO42−, SO42− and oxalate ions.
AB - Abstract— (1) Swelling of synaptosomes was measured spectrophotometrically by recording changes in extinction at 520 nm. (2) Synaptosomes behaved as osmometers in NaCl solutions. When the tonicity of the medium was changed, synaptosome volume changed in accordance with Boyle and van't Hoff's Law. These changes were reversed on restoring the tonicity of the medium. (3) The rate at which a solute entered the synaptosome was determined from the rate of swelling in the presence of that solute. Permeability of synaptosomes to non‐electrolytes was in the order glucose ≪ glycerol < thiourea = formamide < propylene glycol = dimethylsulphoxide. (4) Synaptosomes were freely permeable to ammonium and acetate ions and impermeable to Ca2+, Mg2+, PO42−, SO42− and oxalate ions.
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U2 - 10.1111/j.1471-4159.1970.tb00535.x
DO - 10.1111/j.1471-4159.1970.tb00535.x
M3 - Article
C2 - 4912311
AN - SCOPUS:0014791582
SN - 0022-3042
VL - 17
SP - 565
EP - 571
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -