Resumen
Inflammation induced in the skin of granulocytopenic rabbits by Escherichia coli was examined. Protein exudation and platelet deposition in lesions were measured with 125I-labeled albumin and 111In-labeled platelets. In granulocytopenic rabbits 104-107 live serum-resistant E. coli induced protein exudation and platelet deposition beginning at 3 hr and then progressing over the next 24 hr to much higher levels than in normal rabbits. These responses were associated with interstitial edema and progressive venous thrombosis in the absence of leukocytes; no such reactions were observed in normal rabbits. No reactions were induced in granulocytopenic rabbits by killed E. coli. Of six E. coli strains tested, all three hemolytic strains induced lesions with four to five times more thrombosis (platelet deposition) than did nonhemolytic strains. Two hemolysin-negative mutants lost most of their thrombogenic activity. All three hemolytic strains had cell-associated hemolysin, but only one of these elaborated appreciable free, filterable hemolysin as well.
Idioma original | English |
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Páginas (desde-hasta) | 925-934 |
Número de páginas | 10 |
Publicación | Journal of Infectious Diseases |
Volumen | 150 |
N.º | 6 |
DOI | |
Estado | Published - 1984 |
Nota bibliográfica
Funding Information:Received for publication May 18, 1984, and in revised form August 7, 1984. This work was supported by grant MA7684 from the Medical Research Council of Canada. Dr. Issekutz is supported in part by grant 00-209 from the Medical Research Council of Canada. We thank Rosemary Sampson for secretarial help and Dr. Irvin Snyder for advice. Please address requests for reprints to Dr. Andrew C. Issekutz, Infectious Disease Research Laboratory, I. W. Killam Hospital, 5850 University Avenue, Halifax, Nova Scotia B3J 309, Canada.
ASJC Scopus Subject Areas
- Immunology and Allergy
- Infectious Diseases