Resumen
Human natural killer antigen-1 (HNK-1) is a carbohydrate epitope associated with sulfoglucuronylglycolipids and glycoproteins. Biochemical analyses have demonstrated associations between the HNK-1 epitope and isoforms of the neural cell adhesion molecule (N-CAM) family. In the cerebellum, HNK-1 is prominently expressed in Purkinje cell dendrites and Golgi cells. Purkinje cell expression of HNK-1 reveals an array of parasagittal stripes and transverse zones. Interestingly, the parasagittal expression pattern of HNK-1 is different from those reported with several other markers such as zebrin II/aldolase C and the small heat shock protein HSP25. N-CAM null knockout mice were used to explore the possible role of the HNK-1/N-CAM interaction during the topographical organization of the cerebellar cortex. N-CAM null mice have no N-CAM immunoreactivity but otherwise the cerebellum appears morphologically normal. Further, in the N-CAM null HNK-1 immunoreactivity is abolished from Purkinje cell dendrites but is retained on Golgi cells and neurons of the cerebellar nuclei. Despite the absence of N-CAM/HNK-1, parasagittal stripes and transverse zones in the cerebellum as revealed by using zebrin II immunocytochemistry appear normal.
Idioma original | English |
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Páginas (desde-hasta) | 117-130 |
Número de páginas | 14 |
Publicación | Journal of Neurocytology |
Volumen | 33 |
N.º | 1 SPEC. ISS. |
DOI | |
Estado | Published - ene. 2004 |
Nota bibliográfica
Funding Information:These studies were supported by grants from the Canadian Institutes of Health Research (RH) and the Medical Research Council of Canada. RVS was supported by a studentship from the CIHR Training Program in Genetics, Child Development and Health.
ASJC Scopus Subject Areas
- Anatomy
- General Neuroscience
- Histology
- Cell Biology