Abnormal HNK-1 expression in the cerebellum of an N-CAM null mouse

Hassan Marzban, Roy V. Sillitoe, Monica Hoy, Seung Hyuk Chung, Victor F. Rafuse, Richard Hawkes

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

44 Citas (Scopus)

Resumen

Human natural killer antigen-1 (HNK-1) is a carbohydrate epitope associated with sulfoglucuronylglycolipids and glycoproteins. Biochemical analyses have demonstrated associations between the HNK-1 epitope and isoforms of the neural cell adhesion molecule (N-CAM) family. In the cerebellum, HNK-1 is prominently expressed in Purkinje cell dendrites and Golgi cells. Purkinje cell expression of HNK-1 reveals an array of parasagittal stripes and transverse zones. Interestingly, the parasagittal expression pattern of HNK-1 is different from those reported with several other markers such as zebrin II/aldolase C and the small heat shock protein HSP25. N-CAM null knockout mice were used to explore the possible role of the HNK-1/N-CAM interaction during the topographical organization of the cerebellar cortex. N-CAM null mice have no N-CAM immunoreactivity but otherwise the cerebellum appears morphologically normal. Further, in the N-CAM null HNK-1 immunoreactivity is abolished from Purkinje cell dendrites but is retained on Golgi cells and neurons of the cerebellar nuclei. Despite the absence of N-CAM/HNK-1, parasagittal stripes and transverse zones in the cerebellum as revealed by using zebrin II immunocytochemistry appear normal.

Idioma originalEnglish
Páginas (desde-hasta)117-130
Número de páginas14
PublicaciónJournal of Neurocytology
Volumen33
N.º1 SPEC. ISS.
DOI
EstadoPublished - ene. 2004

Nota bibliográfica

Funding Information:
These studies were supported by grants from the Canadian Institutes of Health Research (RH) and the Medical Research Council of Canada. RVS was supported by a studentship from the CIHR Training Program in Genetics, Child Development and Health.

ASJC Scopus Subject Areas

  • Anatomy
  • General Neuroscience
  • Histology
  • Cell Biology

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