Acid and alkaline ceramidases of rat tissues

M. W. Spence; S. Beed; H. W. Cook

doi:10.1139/o86-056

Acid and alkaline ceramidases of rat tissues

M. W. Spence, S. Beed, H. W. Cook

Medicine

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49 Citas (Scopus)

Resumen

Ceramidase (N-acylsphingosine deacylase) was measured in rat tissue homogenates with N-[1-¹⁴C]oleoyl- and N-[9,10-³H]palmitoyl-sphingosine. Two activities were observed with pH optima of approximately 5 and 8. The activity at pH 8 exceeded that at pH 5 in all tissue and tissue areas examined, and both activities decreased in the following order: kidney > brain stem > cerebrum > liver > spleen > cardiac muscle > lung > psoas muscle. The distribution of activity is not as tissue specific as that of neutral Mg²⁺-stimulated sphingomyelinase which is enriched in cells of neural origin and thus suggests a less specialized role for the alkaline ceramidase.

Idioma original	English
Páginas (desde-hasta)	400-404
Número de páginas	5
Publicación	Biochemistry and Cell Biology
Volumen	64
N.º	5
DOI	https://doi.org/10.1139/o86-056
Estado	Published - 1986

ASJC Scopus Subject Areas

Biochemistry
Molecular Biology
Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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abstract = "Ceramidase (N-acylsphingosine deacylase) was measured in rat tissue homogenates with N-[1-14C]oleoyl- and N-[9,10-3H]palmitoyl-sphingosine. Two activities were observed with pH optima of approximately 5 and 8. The activity at pH 8 exceeded that at pH 5 in all tissue and tissue areas examined, and both activities decreased in the following order: kidney > brain stem > cerebrum > liver > spleen > cardiac muscle > lung > psoas muscle. The distribution of activity is not as tissue specific as that of neutral Mg2+-stimulated sphingomyelinase which is enriched in cells of neural origin and thus suggests a less specialized role for the alkaline ceramidase.",

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T1 - Acid and alkaline ceramidases of rat tissues

AU - Spence, M. W.

AU - Beed, S.

AU - Cook, H. W.

PY - 1986

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AB - Ceramidase (N-acylsphingosine deacylase) was measured in rat tissue homogenates with N-[1-14C]oleoyl- and N-[9,10-3H]palmitoyl-sphingosine. Two activities were observed with pH optima of approximately 5 and 8. The activity at pH 8 exceeded that at pH 5 in all tissue and tissue areas examined, and both activities decreased in the following order: kidney > brain stem > cerebrum > liver > spleen > cardiac muscle > lung > psoas muscle. The distribution of activity is not as tissue specific as that of neutral Mg2+-stimulated sphingomyelinase which is enriched in cells of neural origin and thus suggests a less specialized role for the alkaline ceramidase.

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Acid and alkaline ceramidases of rat tissues

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