Aggregation and particle formation of tRNA by dendrimers

Emilie Froehlich, Jean Sebastian Mandeville, Laurent Kreplak, Heidar Ali Tajmir-Riahi

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

19 Citas (Scopus)

Resumen

Major attention has been focused on dendrimer-DNA complexes because of their applications in gene delivery systems. Dendrimers are also used to transport miRNA and siRNA in vitro. We examine the interaction of tRNA with several dendrimers of different compositions, mPEG-PAMAM (G3), mPEG-PAMAM (G4), and PAMAM (G4) under physiological conditions using constant tRNA concentration and various dendrimer contents. FTIR, UV-visible, and CD spectroscopic methods as well as atomic force microscopy (AFM) were used to analyze the macromolecule binding mode, the binding constant, and the effects of dendrimer complexation on RNA stability, aggregation, particle formation, and conformation. Structural analysis showed that dendrimer-tRNA complexation occurred via RNA bases and the backbone phosphate group with both hydrophilic and hydrophobic contacts. The overall binding constants of K mPEG-G3 = 7.6 (±0.9) × 10 3 M -1, K mPEG-G4 = 1.5 (±0.40) × 10 4 M -1, and K PAMAM-G4 = 5.3 (±0.60) × 10 4 M -1 show stronger polymer-RNA complexation by PAMAM-G4 than pegylated dendrimers. RNA remains in the A-family structure, whereas biopolymer aggregation and particle formation occurred at high polymer concentrations.

Idioma originalEnglish
Páginas (desde-hasta)2780-2787
Número de páginas8
PublicaciónBiomacromolecules
Volumen12
N.º7
DOI
EstadoPublished - jul. 11 2011

ASJC Scopus Subject Areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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