Anti-DNA activity of IgG F(ab')2 from normal human serum

R. L. Rubin, R. I. Carr

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Resumen

The components of normal human serum (NHS) which bound DNA in a standard assay for anti-DNA antibody were characterized. IgG was the major detectable protein isolated from NHS by affinity chromatography on DNA-cellulose. A second adsorption of the whole serum IgG with DNA-cellulose did not remove additional γ-globulin indicating that only a very small fraction of the IgG was capable of binding DNA. This binding activity was largely restricted to denatured DNA. IgG (Fab')2 bound DNA as well as the intact molecules demonstrating the antibody-like nature of the IgG binding. These results suggest that IgG antibody to denatured DNA is a normal component of human serum.

Idioma originalEnglish
Páginas (desde-hasta)1604-1607
Número de páginas4
PublicaciónJournal of Immunology
Volumen122
N.º4
EstadoPublished - 1979
Publicado de forma externa

ASJC Scopus Subject Areas

  • Immunology and Allergy
  • Immunology

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