Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)

Madhury R. Saha; Neil W. Ross; Rolf E. Olsen; Santosh P. Lall

doi:10.1016/j.cbpb.2006.04.011

Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)

Madhury R. Saha, Neil W. Ross, Rolf E. Olsen, Santosh P. Lall

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

10 Citas (Scopus)

Resumen

A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R² = 0.80-0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336-343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh. Crown

Idioma original	English
Páginas (desde-hasta)	488-495
Número de páginas	8
Publicación	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volumen	144
N.º	4
DOI	https://doi.org/10.1016/j.cbpb.2006.04.011
Estado	Published - ago. 2006
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
This research was funded by the Institute of Marine Research, Bergen, Norway and the National Research Council, Canada. Madhury Saha received partial funding from Dalhousie University Faculty of Graduate Studies Scholarship program. The assistance of Prabir Roy, Sean Tibbetts and Joyce Milley is gratefully acknowledged. The authors thank Dr. Vanya Ewart for the review of the manuscript.

ASJC Scopus Subject Areas

Biochemistry
Physiology
Aquatic Science
Animal Science and Zoology
Molecular Biology

PubMed: MeSH publication types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Acceder al documento

10.1016/j.cbpb.2006.04.011

Otros archivos y enlaces

Citar esto

Saha, M. R., Ross, N. W., Olsen, R. E., & Lall, S. P. (2006). Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 144(4), 488-495. https://doi.org/10.1016/j.cbpb.2006.04.011

Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). / Saha, Madhury R.; Ross, Neil W.; Olsen, Rolf E. et al.
En: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 144, N.º 4, 08.2006, p. 488-495.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

Saha, MR, Ross, NW, Olsen, RE & Lall, SP 2006, 'Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 144, n.º 4, pp. 488-495. https://doi.org/10.1016/j.cbpb.2006.04.011

@article{59515686883f4c74a230f96cecbd1cd7,

title = "Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)",

abstract = "A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80-0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336-343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh. Crown",

author = "Saha, {Madhury R.} and Ross, {Neil W.} and Olsen, {Rolf E.} and Lall, {Santosh P.}",

note = "Funding Information: This research was funded by the Institute of Marine Research, Bergen, Norway and the National Research Council, Canada. Madhury Saha received partial funding from Dalhousie University Faculty of Graduate Studies Scholarship program. The assistance of Prabir Roy, Sean Tibbetts and Joyce Milley is gratefully acknowledged. The authors thank Dr. Vanya Ewart for the review of the manuscript.",

year = "2006",

month = aug,

doi = "10.1016/j.cbpb.2006.04.011",

language = "English",

volume = "144",

pages = "488--495",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)

AU - Saha, Madhury R.

AU - Ross, Neil W.

AU - Olsen, Rolf E.

AU - Lall, Santosh P.

N1 - Funding Information: This research was funded by the Institute of Marine Research, Bergen, Norway and the National Research Council, Canada. Madhury Saha received partial funding from Dalhousie University Faculty of Graduate Studies Scholarship program. The assistance of Prabir Roy, Sean Tibbetts and Joyce Milley is gratefully acknowledged. The authors thank Dr. Vanya Ewart for the review of the manuscript.

PY - 2006/8

Y1 - 2006/8

N2 - A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80-0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336-343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh. Crown

AB - A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80-0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336-343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh. Crown

UR - http://www.scopus.com/inward/record.url?scp=33746296564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746296564&partnerID=8YFLogxK

U2 - 10.1016/j.cbpb.2006.04.011

DO - 10.1016/j.cbpb.2006.04.011

M3 - Article

C2 - 16781177

AN - SCOPUS:33746296564

SN - 1096-4959

VL - 144

SP - 488

EP - 495

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 4

ER -

Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto