Bile salt-activated lipase expression during larval development in the haddock (Melanogrammus aeglefinus)

J. C. Perez-Casanova, H. M. Murray, J. W. Gallant, N. W. Ross, S. E. Douglas, S. C. Johnson

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

35 Citas (Scopus)

Resumen

In the present work, we report the characterization of the partial cDNA sequence of a bile salt-activated lipase (BAL) cDNA from haddock. The predicted polypeptide encoded by the cDNA sequence contains the bile salt-binding site characteristic of all BALs at amino acid positions 36-45, and the lipid-binding site thus far only reported in fish BALs starting at position 345. Other features of BAL are also present including: the active site serine motif at positions 111-117, the catalytic triad formed by the residues S114, D239 and H358, and an N-glycosylation site at position 107. The relative levels of BAL gene expression were determined in haddock larvae during ontogeny by reverse transcription-polymerase chain reaction (RT-PCR) with the earliest detectable transcript levels identified at hatch. Using in situ hybridization, the BAL transcripts were localized consistently in the pancreas of haddock larvae from mouth opening until 401 degree days (DD). Using biochemical techniques, the specific activity of BAL was found to decline significantly over time. Our results also suggest that haddock larvae are capable of digesting lipids at the time of mouth opening. Crown

Idioma originalEnglish
Páginas (desde-hasta)601-617
Número de páginas17
PublicaciónAquaculture
Volumen235
N.º1-4
DOI
EstadoPublished - jun. 1 2004
Publicado de forma externa

Nota bibliográfica

Funding Information:
We thank Kelly Soanes for the critical review of this manuscript. This research has been supported in part by funds from the AquaNet, Network Centres of Excellence, Heritage Aquaculture and the Fundacion Pablo Garcia. This is NRCC publication number 42432.

ASJC Scopus Subject Areas

  • Aquatic Science

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