Carvacrol exhibits rapid bactericidal activity against Streptococcus pyogenes through cell membrane damage

Niluni M. Wijesundara, Song F. Lee, Zhenyu Cheng, Ross Davidson, H. P.Vasantha Rupasinghe

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

104 Citas (Scopus)

Resumen

Streptococcus pyogenes is an important human pathogen worldwide. The identification of natural antibacterial phytochemicals has renewed interest due to the current scarcity of antibiotic development. Carvacrol is a monoterpenoid found in herbs. We evaluated carvacrol alone and combined with selected antibiotics against four strains of S. pyogenes in vitro. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of carvacrol against S. pyogenes were 125 µg/mL (0.53 mM) and 250 µg/mL (1.05 mM), respectively. Kill curve results showed that carvacrol exhibits instantaneous bactericidal activity against S. pyogenes. We also demonstrated the potential mechanism of action of carvacrol through compromising the cell membrane integrity. Carvacrol induced membrane integrity changes leading to leakage of cytoplasmic content such as lactate dehydrogenase enzymes and nucleic acids. We further confirmed dose-dependent rupturing of cells and cell deaths using transmission electron microscopy. The chequerboard assay results showed that carvacrol possesses an additive-synergistic effect with clindamycin or penicillin. Carvacrol alone, combined with clindamycin or penicillin, can be used as a safe and efficacious natural health product for managing streptococcal pharyngitis.

Idioma originalEnglish
Número de artículo1487
PublicaciónScientific Reports
Volumen11
N.º1
DOI
EstadoPublished - dic. 2021

Nota bibliográfica

Funding Information:
Authors thankfully recognize funding from the Collaborative Research and Development Grant program (CRDPJ 448052) of the Natural Sciences and Engineering Research Council (NSERC) of Canada and Island Abbey Foods, Charlottetown, PE, Canada.

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus Subject Areas

  • General

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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