Chromosome level reference of Atlantic halibut Hippoglossus hippoglossus provides insight into the evolution of sexual determination systems

Anthony L. Einfeldt, Tony Kess, Amber Messmer, Steven Duffy, Brendan F. Wringe, Jonathan Fisher, Cornelia den Heyer, Ian R. Bradbury, Daniel E. Ruzzante, Paul Bentzen

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

22 Citas (Scopus)

Resumen

Changes in the genetic mechanisms that control sexual determination have occurred independently across the tree of life, and with exceptional frequency in teleost fishes. To investigate the genomic changes underlying the evolution of sexual determination, we sequenced a chromosome-level genome, multitissue transcriptomes, and reduced representation population data for the Atlantic halibut (Hippoglossus hippoglossus), which has an XY/XX sex determination mechanism and has recently diverged (0.9–3.8 Ma) from the Pacific halibut (Hippoglossus stenolepis), which has a ZZ/ZW system. We used frequency and coverage-based population approaches to identify a putative sex-determining factor, GSDF. We characterized regions with elevated heterozygosity and linkage disequilibrium indicating suppression of recombination across a nascent sex chromosome. We detected testis-specific expression of GSDF, the sequence of which is highly conserved across flatfishes. Based on evidence from genome-wide association, coverage, linkage disequilibrium, testis and brain transcriptomes, and sequence conservation with other flatfishes, we propose a mechanism for the recent evolution of an XY sex-determination mechanism in Atlantic halibut. Changes to the ancestral sex-determining gene DMRT1 in regulating the downstream gene GSDF probably coincided with GSDF, or a proximal regulatory element of it, becoming the primary sex-determining factor. Our results suggest changes to a small number of elements can have drastic repercussions for the genomic substrate available to sex-specific evolutionary forces, providing insight into how certain elements repeatedly evolve to control sex across taxa. Our chromosome-level assembly, multitissue transcriptomes, and population genomic data provide a valuable resource and understanding of the evolution of sexual systems in fishes.

Idioma originalEnglish
Páginas (desde-hasta)1686-1696
Número de páginas11
PublicaciónMolecular Ecology Resources
Volumen21
N.º5
DOI
EstadoPublished - jul. 2021

Nota bibliográfica

Funding Information:
The fish used for genome sequencing was provided by Gerald Johnson at Halibut PEI. DNA extraction and sequencing was coordinated by Olivier Fedrigo at VGP Rockefeller. Genome curation was by Joanna Collins, and the gEVAL database was generated by William Chow at Wellcome Sanger Institute. We would also like to thank: Bruce Chapman, Atlantic Halibut Council; Gerry and Gary Dedrick, Shelburne County Quota Group; Lori Baker, Eastern Shore Fisherman's Protective Association; Erin Carruthers and Miranda McGrath, FFAW-Unifor; Albert Moore, Dylan Buchannan, and Fisheries Observers, Javitech Atlantic; Derrick Lambert and Fisheries Observers, Seawatch Inc.; Mathieu Desgagnés, Hugo Bourdages, and the DFO Science staff and crew of the CCGS Teleost; and Jamie Emberley, DFO Science staff, and the crew of the CCGS Alfred Needler.

Publisher Copyright:
© 2021 John Wiley & Sons Ltd

ASJC Scopus Subject Areas

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

PubMed: MeSH publication types

  • Journal Article

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