Comparative Evaluation of Luminex xTAG® Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of C. difficile Toxin tcdB in Stool Samples from a Pediatric Population

Hannah Tyrrell; Sarah B.N. Morin; Colin D. Lloyd; Brendon Parsons; Taryn Stokowski; Jianling Xie; Ran Zhuo; Bonita E. Lee; Xiao Li Pang; Stephen B. Freedman; Linda Chui

doi:10.3390/microorganisms10112214

Comparative Evaluation of Luminex xTAG^® Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of C. difficile Toxin tcdB in Stool Samples from a Pediatric Population

Hannah Tyrrell, Sarah B.N. Morin, Colin D. Lloyd, Brendon Parsons, Taryn Stokowski, Jianling Xie, Ran Zhuo, Bonita E. Lee, Xiao Li Pang, Stephen B. Freedman, Linda Chui

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5 Citas (Scopus)

Resumen

Detection of Clostridioides difficile toxins in patients with gastroenteritis has increasingly been accomplished through the use of enteric multiplex syndromic panels. Comparisons of the performance of these panels to both direct-from-stool (DFS) and culture-enriched stools followed by polymerase chain reaction (PCR) methods in pediatric populations are limited. Here, we compare the performance of the Luminex xTAG^® Gastrointestinal Pathogen Panel (GPP) to our DFS in-house real-time PCR (DFS RT-PCR) assay for the detection of C. difficile toxin gene, tcdB, using 2641 stool specimens collected from children enrolled in the Alberta Provincial Pediatric EnTeric Infection Team (APPETITE) study in Alberta, Canada. We used culture enrichment followed by in-house RT-PCR to resolve discordant results between the two assays. We found excellent agreement (k = 0.89) between the GPP and our DFS RT-PCR assay: the positive percent agreement between the two assays was 97%, and the negative percent agreement was 99%. GPP, a multi-analyte platform can easily be implemented into a routine diagnostic laboratory for detecting enteric pathogens including C. difficile.

Idioma original	English
Número de artículo	2214
Publicación	Microorganisms
Volumen	10
N.º	11
DOI	https://doi.org/10.3390/microorganisms10112214
Estado	Published - nov. 2022
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
This work was supported by the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE), which was funded by a grant from the Alberta Innovates–Health Solutions Team Collaborative Research Innovation Opportunity. APPETITE is also supported through a partnership awards by the Alberta Children’s Hospital Research Institute (Calgary, AB, Canada) and the Women and Children’s Hospital Research Institute (Edmonton, AB, Canada). SBF is supported by the Alberta Children’s Hospital Foundation Professorship in Child Health and Wellness. The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children’s Hospital Foundation. This study was also supported by Alberta Health Services Residual Funds. HT was supported by Alberta Innovates Summer Research Studentship; CL was the recipient of the Canada Graduate Scholarship, Walter H. Johns Graduate Fellowship and Women and Children’s Hospital Research Institute Graduate Studentship.

Publisher Copyright:
© 2022 by the authors.

ASJC Scopus Subject Areas

Microbiology
Microbiology (medical)
Virology

PubMed: MeSH publication types

Journal Article

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10.3390/microorganisms10112214

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Tyrrell, H., Morin, S. B. N., Lloyd, C. D., Parsons, B., Stokowski, T., Xie, J., Zhuo, R., Lee, B. E., Pang, X. L., Freedman, S. B., & Chui, L. (2022). Comparative Evaluation of Luminex xTAG^® Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of C. difficile Toxin tcdB in Stool Samples from a Pediatric Population. Microorganisms, 10(11), Artículo 2214. https://doi.org/10.3390/microorganisms10112214

Comparative Evaluation of Luminex xTAG^® Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of C. difficile Toxin tcdB in Stool Samples from a Pediatric Population. / Tyrrell, Hannah; Morin, Sarah B.N.; Lloyd, Colin D. et al.
En: Microorganisms, Vol. 10, N.º 11, 2214, 11.2022.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

Tyrrell, H, Morin, SBN, Lloyd, CD, Parsons, B, Stokowski, T, Xie, J, Zhuo, R, Lee, BE, Pang, XL, Freedman, SB & Chui, L 2022, 'Comparative Evaluation of Luminex xTAG^® Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of C. difficile Toxin tcdB in Stool Samples from a Pediatric Population', Microorganisms, vol. 10, n.º 11, 2214. https://doi.org/10.3390/microorganisms10112214

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title = "Comparative Evaluation of Luminex xTAG{\textregistered} Gastrointestinal Pathogen Panel and Direct-From-Stool Real-Time PCR for Detection of C. difficile Toxin tcdB in Stool Samples from a Pediatric Population",

abstract = "Detection of Clostridioides difficile toxins in patients with gastroenteritis has increasingly been accomplished through the use of enteric multiplex syndromic panels. Comparisons of the performance of these panels to both direct-from-stool (DFS) and culture-enriched stools followed by polymerase chain reaction (PCR) methods in pediatric populations are limited. Here, we compare the performance of the Luminex xTAG{\textregistered} Gastrointestinal Pathogen Panel (GPP) to our DFS in-house real-time PCR (DFS RT-PCR) assay for the detection of C. difficile toxin gene, tcdB, using 2641 stool specimens collected from children enrolled in the Alberta Provincial Pediatric EnTeric Infection Team (APPETITE) study in Alberta, Canada. We used culture enrichment followed by in-house RT-PCR to resolve discordant results between the two assays. We found excellent agreement (k = 0.89) between the GPP and our DFS RT-PCR assay: the positive percent agreement between the two assays was 97%, and the negative percent agreement was 99%. GPP, a multi-analyte platform can easily be implemented into a routine diagnostic laboratory for detecting enteric pathogens including C. difficile.",

author = "Hannah Tyrrell and Morin, {Sarah B.N.} and Lloyd, {Colin D.} and Brendon Parsons and Taryn Stokowski and Jianling Xie and Ran Zhuo and Lee, {Bonita E.} and Pang, {Xiao Li} and Freedman, {Stephen B.} and Linda Chui",

note = "Funding Information: This work was supported by the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE), which was funded by a grant from the Alberta Innovates–Health Solutions Team Collaborative Research Innovation Opportunity. APPETITE is also supported through a partnership awards by the Alberta Children{\textquoteright}s Hospital Research Institute (Calgary, AB, Canada) and the Women and Children{\textquoteright}s Hospital Research Institute (Edmonton, AB, Canada). SBF is supported by the Alberta Children{\textquoteright}s Hospital Foundation Professorship in Child Health and Wellness. The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children{\textquoteright}s Hospital Foundation. This study was also supported by Alberta Health Services Residual Funds. HT was supported by Alberta Innovates Summer Research Studentship; CL was the recipient of the Canada Graduate Scholarship, Walter H. Johns Graduate Fellowship and Women and Children{\textquoteright}s Hospital Research Institute Graduate Studentship. Publisher Copyright: {\textcopyright} 2022 by the authors.",

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