Cryoglobulinemia and decreased monocyte chemotaxis in malignant melanoma

D. A. Norris; J. C. Huff; J. M. Swinehart; R. I. Carr; E. G. Thorne; W. L. Weston; R. M. McIntosh

doi:10.1111/1523-1747.ep12523190

Cryoglobulinemia and decreased monocyte chemotaxis in malignant melanoma

D. A. Norris, J. C. Huff, J. M. Swinehart, R. I. Carr, E. G. Thorne, W. L. Weston, R. M. McIntosh

Medicine

Medicine

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5 Citas (Scopus)

Resumen

The sera from 13 patients with malignant melanoma were evaluated for immune complexes by cryoprecipitation and the ¹²⁵I Clq binding assay. Cryoprecipitates were identified in 12/13 patients (92%) and cryoimmunoglobulins in 7/13 patients (54%). Either cryoimmunoglobulin or elevated binding was identified in 8/13 patients (62%). Incubation of normal monocytes with the resuspended cryoimmunoglobulin of 7 melanoma patients produced greater than 50% reduction in the ability of the monocytes to respond to chemotactic stimuli (p <0.01). Similar inhibition was seen with cryoimmunoglobulin from erythema multiforme patients, but not with medium alone, albuminm heat aggregated albumin or heat aggregatedIgG in similar concentrations. No soluble factors produced in vitro could be demonstrated to produce this inhibition. Inhibition of monocyte function by immune complexes may be an important component of impaired host response to malignant melanoma, or alternatively may represent an important mechanism for the accumulation of monocytes at sites of inflammation analogous to migration inhibition factor.

Idioma original	English
Páginas (desde-hasta)	219-223
Número de páginas	5
Publicación	Journal of Investigative Dermatology
Volumen	75
N.º	3
DOI	https://doi.org/10.1111/1523-1747.ep12523190
Estado	Published - 1980

Nota bibliográfica

Funding Information:
The Hellstroms and their associates first documented that the cell mediated immune response to melanoma tumor cells could be abrogated by antigen-antibody complexes from the sera of progressing melanoma patients [ 4-6]. Subsequent studies have shown that such "blocking" of tumor cytotoxicity can be caused by antibody, antigen, antigen-antibody complexes or Manuscript received December 18, 1 978; February 4, 1980 This work was supported by the Antonoff Cancer Research Grant, the NIH Clinical Investigator Award l-l<OS-AM00510-01, a program grant from the National Cancer Institute CA 12247, and Mycosis Fungoicles Grant 5-ROl-AI-13171-03. Reprint requests to: David A. Norris, M.D., Department of Dermatology, University of Colorado Medical Center, 4200 East Ninth Avenue, Denver, Colorado 80262. Abbreviations: AGG: aggregated gamma globulin CLq BA: CLq binding activity PBS: phosphate-buffered saline PEG: polyethylene glycol RF: rheumatoid factor

ASJC Scopus Subject Areas

Biochemistry
Molecular Biology
Dermatology
Cell Biology

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abstract = "The sera from 13 patients with malignant melanoma were evaluated for immune complexes by cryoprecipitation and the 125I Clq binding assay. Cryoprecipitates were identified in 12/13 patients (92%) and cryoimmunoglobulins in 7/13 patients (54%). Either cryoimmunoglobulin or elevated binding was identified in 8/13 patients (62%). Incubation of normal monocytes with the resuspended cryoimmunoglobulin of 7 melanoma patients produced greater than 50% reduction in the ability of the monocytes to respond to chemotactic stimuli (p <0.01). Similar inhibition was seen with cryoimmunoglobulin from erythema multiforme patients, but not with medium alone, albuminm heat aggregated albumin or heat aggregatedIgG in similar concentrations. No soluble factors produced in vitro could be demonstrated to produce this inhibition. Inhibition of monocyte function by immune complexes may be an important component of impaired host response to malignant melanoma, or alternatively may represent an important mechanism for the accumulation of monocytes at sites of inflammation analogous to migration inhibition factor.",

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N2 - The sera from 13 patients with malignant melanoma were evaluated for immune complexes by cryoprecipitation and the 125I Clq binding assay. Cryoprecipitates were identified in 12/13 patients (92%) and cryoimmunoglobulins in 7/13 patients (54%). Either cryoimmunoglobulin or elevated binding was identified in 8/13 patients (62%). Incubation of normal monocytes with the resuspended cryoimmunoglobulin of 7 melanoma patients produced greater than 50% reduction in the ability of the monocytes to respond to chemotactic stimuli (p <0.01). Similar inhibition was seen with cryoimmunoglobulin from erythema multiforme patients, but not with medium alone, albuminm heat aggregated albumin or heat aggregatedIgG in similar concentrations. No soluble factors produced in vitro could be demonstrated to produce this inhibition. Inhibition of monocyte function by immune complexes may be an important component of impaired host response to malignant melanoma, or alternatively may represent an important mechanism for the accumulation of monocytes at sites of inflammation analogous to migration inhibition factor.

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Cryoglobulinemia and decreased monocyte chemotaxis in malignant melanoma

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

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