CXCR3 is required for migration to dermal inflammation by normal and in vivo activated T cells: Differential requirements by CD4 and CD8 memory subsets

Karkada Mohan, Erin Cordeiro, Maria Vaci, Christopher McMaster, Thomas B. Issekutz

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

48 Citas (Scopus)

Resumen

Lymphocytes in inflamed tissues express numerous chemokine receptors. The relative importance of these receptors for migration in inflammation is unclear. The role of CXCR3 in T cell subset migration was examined using monoclonal antibodies developed to rat CXCR3. CXCR3 was expressed on sixfold more CD8+ (∼30%) than CD4+ (∼5%) T cells in spleen, lymph nodes and blood, and on ∼10% of CD4+CD45RC- (memory) and ∼50% of CD8+CD45RC+ spleen T cells. After immunization, CXCR3 increased tenfold on CD4+ lymph node lymphoblasts (∼55%), and >90% of inflammatory exudate T cells were CXCR3+. CXCR3+ T cells migrated significantly better than CXCR3- T cells to all dermal inflammatory stimuli tested in vivo, even though these T cells are a minority of the memory T cells. Blocking CXCR3 inhibited recruitment of 60-85% of unstimulated T cells and up to 90% of CD8+CD45RC+ effector T cells, but caused <50% inhibition of CD4+ and CD8+ memory (CD45RC-) T cells. About 90% of T lymphoblast migration to IFN-γ, IFN-γ plus TNF-α, polyinosinic polycytidylic acid, lipopolysaccharide, and delayed-type hypersensitivity (DTH)-induced inflammation was inhibited. Blockade also reduced DTH-induced induration. Thus, CXCR3 has a non-redundant role in T cell migration to dermal inflammation and is critical for activated T lymphoblast recruitment, but memory T cells are less dependent on CXCR3 for their infiltration.

Idioma originalEnglish
Páginas (desde-hasta)1702-1711
Número de páginas10
PublicaciónEuropean Journal of Immunology
Volumen35
N.º6
DOI
EstadoPublished - jun. 2005

ASJC Scopus Subject Areas

  • Immunology and Allergy
  • Immunology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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