Derivatization of bile acids with taurine for analysis by fast atom bombardment mass spectrometry with collision-induced fragmentation

J. Zhang, W. J. Griffiths, T. Bergman, J. Sjovall

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

33 Citas (Scopus)

Resumen

When analyzed by fast atom bombardment mass spectrometry, taurine- conjugated bile acids give intense [M-H]- pseudomolecular ions that can be subjected to collision-induced fragmentation to give structural information. A method has been developed that permits rapid coupling of taurine to unconjugated, glycine-conjugated, sulfated, and glucuronidated bile acids. The reaction is performed for 2 h at room temperature in aqueous pyridine hydrochloride buffer, with or without dioxane, using 0.1 M 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide as the coupling agent and 0.2 M taurine. The yields are higher than 95%. In contrast to published coupling reactions, the method permits conjugation of bile acids with the labile 7α-hydroxy-3- oxo-4-ene structure.

Idioma originalEnglish
Páginas (desde-hasta)1895-1900
Número de páginas6
PublicaciónJournal of Lipid Research
Volumen34
N.º11
EstadoPublished - 1993
Publicado de forma externa

ASJC Scopus Subject Areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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