Differential effects of uniaxial and biaxial strain on U937 macrophage-like cell morphology: Influence of extracellular matrix type proteins

Tissue engineering concepts have expanded in the last decade to consider the importance of biochemical signaling from extracellular matrix (ECM) proteins adhered to substrates such as polymeric and ceramic scaffolds. This study investigated combined ECM/mechanical factors on the key signaling cells (macrophages) for wound healing, since previously, mechanical strain and ECM proteins have only been considered separately for their effects on macrophage morphology. Human U937 macrophage-like cells were cultured on a model elastomeric membrane, coated with either collagen type I or poly-RGD peptide (ProNectin®). The cells were subjected to cyclic uniform uniaxial or nonuniform biaxial strain with the Flexercell™ Tension Plus system to simulate strains that various soft tissue implants may undergo during the critical tissue-implant integration period. The surface coatings affected total cellular protein, which was significantly higher in cells on collagen than ProNectin® coated surfaces after biaxial, but not uniaxial strain, whereas ProNectin® coated surfaces caused a decrease in DNA following uniaxial, but not biaxial strain. Adding the protein coatings that relate to the wound healing process during tissue regeneration, elicited effects specific to the strain type imposed. The combination of these parameters caused changes in U937 macrophage-like cells that should be considered in the outcome of the desired performance in the tissue-material constructs.