Effect of schisanhenol on function and surface shape of rat neutrophils

Aim: To study the regulatory effect of Schisanhenol (Sal) on function of rat neutrophils. Results: It was found that Sal (1, 10, and 100 μmol·L^-1) inhibited neutrophil activities such as chemotaxis, phagocytosis, and superoxide anion production in vitro in a concentration-dependent manner. Changes of surface morphology of neutrophils were observed by scanning electron microscopy, showing that the ruffles and pseudopods on neutrophil surface increased under the stimulation by chemotactic peptide N-formyl-Met-Leu-Phe (FMLP). When pretreated with Sal 100 μmol ·L^-1, the ruffles and pseudopods disappeared and the surface became smooth. Sal 100 μmol·L^-1 decreased the cytosolic calcium concentration of neutrophils and increased the intracellular cAMP level. Conclusion: These data suggested that Sal could inhibit the function of rat neutrophils through affecting the cytosolic free calcium and cAMP level besides its antioxidant activity.