Effects of Sinefungin on rRNA production and methylation in the yeast Saccharomyces cerevisiae

W. Li Audrey, Richard A. Singer, Gerald C. Johnston

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

7 Citas (Scopus)

Resumen

The antifungal agent, Sinefungin (SF), has been shown to be an inhibitor of transmethylation reactions. We report here the effects of SF on the production and methylation of rRNA in the yeast, Saccharomyces cerevisiae. Under conditions of SF treatment which have been shown to affect the regulation of cell proliferation in this yeast, pulse-chase labeling experiments using [methul-3H]methionine and [3H]uracil indicated that methyl incorporation into rRNA during a short labeling period was inhibited, and stable 18 S rRNA production was differentially decreased. Other experiments quantitating modified nucleotides in newly produced rRNA showed that stable molecules were methylated. Taken together, these results suggest that SF slows methylation of rRNA, and is associated with differential loss of undermethylated 18 S rRNA Species.

Idioma originalEnglish
Páginas (desde-hasta)613-620
Número de páginas8
PublicaciónArchives of Biochemistry and Biophysics
Volumen240
N.º2
DOI
EstadoPublished - ago. 1 1985

ASJC Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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